fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

Artigo - Open Access.

Idioma principal

PRODUCTION OF ANTIOXIDANT HYDROLYSATE FROM BOVINE CASEIN USING IMMOBILIZED PENICILLIUM AURANTIOGRISEUM PROTEASE ON MAGNETIC NANOPARTICLES

DUARTE NETO, J. M. W. ; MACIEL, J. C.; CARVALHO JR, L. B.; LIMA, C. A.; PORTO, A. L. F.;

Artigo:

Peptides with bioactive properties can be released from the precursor proteins during food processing or digestion, and act as antioxidative compounds. Although, it has been proven that hydrolysate exerts higher antioxidant activity than purified peptides. The process of enzyme immobilization proved to be more practical and advantageous for catalysis than using free enzyme. The aim of this study was to use a protease produced by Penicillium aurantiogriseum immobilized on polianilin-coated magnetic nanoparticles, for bovine casein hydrolysis and to evaluate the hydrolysate antioxidant properties. The 60-80% saline fraction of crude extract was used in immobilization. Commercial casein from bovine milk 1% (w/v) was hydrolyzed by 10 mg/mL of immobilized protease. The casein hydrolysates were used in ABTS and H2O2 scavenging activity assays, where Trolox was used as standard. In the ABTS scavenging assay, the hydrolysate (1% w/v) revealed a 1341 µmol of Trolox equivalent activity and in the hydrogen peroxide scavenging assay, the hydrolysate (using 0.1% w/v) presented a 547.82 µmol of Trolox equivalent activity. In both assays the hydrolysates presented a more than 2.5 times greater activity than non-hydrolyzed casein, which validates the process capacity of developing casein derived natural antioxidant ingredients with potential for functional foods.

Artigo:

Palavras-chave:

DOI: 10.5151/chemeng-cobeq2014-0182-26654-174176

Referências bibliográficas
  • [1] ABIDI, F.; AISSAOUI, N.; GAUDIN, J-C.; CHOBERT, J-M.; HAERTLÉ, T.; MARZOUKI, M.N. MS Analysis and Molecular Characterization of Botrytis cinerea Protease Prot-2. Use in Bioactive Peptides Production. Appl. Biochem. Biotechnol., v. 170 (2), p. 231-47, 2013.
  • [2] CORRÊA, A.P.F.; DAROIT, D.J.; COELHO, J.; MEIRA, S.M.M.; LOPES, F.C.; SEGALIN, J.; RISSO, P.H.; BRANDELLI, A. Antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease. J. Sci. Food. Agric., v.91, p. 2247–54, 2011.
  • [3] DUAN, X.; OCEN, D.; WU, F.; LI, M.; YANG, N.; XU, J.; CHEN, H.; HUANG, L.; JIN, Z.; XU, X. Purification and characterization of a natural antioxidant peptide from fertilized eggs. Food. Res. Int. v. 56, p. 18–24, 201
  • [4] GIRGIH, A.T.; HE, R.; MALOMO, S.; OFFENGENDEN, M.; WU, J.; ALUKO, R.E. Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides. J. Funct. Foods., v.1, p. 1–11, 2013.
  • [5] GUO, Y.; ZHANG, T.; JIANG, B.; MIAO, M.; MU, W. The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines. J. Funct. Foods., p. 1–8, 2014.
  • [6] GRAMINHO, E.R.; DA SILVA, R.R.; DE FREITAS CABRAL, T.P.; ARANTES, E.C.; DA ROSA, N.G.; JULIANO, L.; OKAMOTO, D.N.; DE OLIVEIRA, L.C.G.; KONDO, M.Y.; JULIANO, M.A.; CABRAL, H. Purification, characterization, and specificity determination Área temática: Engenharia e Tecnologia de Alimentos 6of a new serine protease secreted by Penicillium waksmanii. Appl. Biochem. Biotechnol., v. 169, p. 201–14, 2013.
  • [7] INTARASIRISAWAT, R.; BENJAKUL, S.; WU, J.; VISESSANGUAN, W. Isolation of antioxidative and ACE inhibitory peptides from protein hydrolysate of skipjack (Katsuwana pelamis) roe. J. Funct. Foods., v. 5, p. 1854–1862, 2013.
  • [8] KIM, E-K.; OH, H-J.; KIM, Y-S.; HWANG, J-W.; AHN, C-B.; LEE, J.S.; JEON, Y-J.; MOON, S-H.; SUNG, S.H.; JEON, B-T.; PARK, P-J. Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties. Fish. Shellfish. Immunol., v. 34, p. 1078–84, 2013.
  • [9] KITTIPHATTANABAWON, P.; BENJAKUL, S.; VISESSANGUAN, W.; SHAHIDI, F. Gelatin hydrolysate from blacktip shark skin prepared using papaya latex enzyme: Antioxidant activity and its potential in model systems. Food. Chem., v. 135, p. 1118–26, 2012.
  • [10] LIMA, C.A.; MARQUES, D.A.V.; BARROS NETO, B.; LIMA FILHO, J.L.; CARNEIRO-DA-CUNHA, M.G.; PORTO, A.L.F. Fermentation medium for collagenase production by Penicillium aurantiogriseum URM4622. Biotechnol. Prog., v. 27, p. 1470–7, 2011.
  • [11] LIMA, C.A.; FILHO, J.L.L.; NETO, B.B.; CONVERTI, A.; CUNHA, M.G.C.DA.; PORTO, A.L.F. Production and Characterization of a Collagenolytic Serine Protease by Penicillium aurantiogriseum URM 4622: A Factorial Study. Biotechnol. Bioprocess. Eng., 20
  • [12] MURO URISTA, C.; ÁLVAREZ FERNÁNDEZ, R.; RIERA RODRIGUEZ, F.; ARANA CUENCA, A.; TÉLLEZ JURADO, A. Review: Production and functionality of active peptides from milk. Food. Sci. Technol. Int., v. 17, p. 293–317, 2011.
  • [13] NERI, D.F.M.; BALCÃO, V.M.; COSTA, R.S.; FERREIRA, E.M.F.C.; TORRES, D.P.M.; RODRIGUES, L.R.; JR, L.B.C.; TEIXEIRA, J.A. B-Galactosidase from Aspergillus oryzae immobilized onto different magnetic supports : A comparative experimental and modelling study of the galactooligosaccharides production. p. 1036–1041, 2008.
  • [14] PIHLANTO, A. Antioxidative peptides derived from milk proteins. Int. Dairy. J., v. 16, p. 1306–1314, 2006.
  • [15] RE, R.; PELLEGRINI, N.; PROTEGGENTE, A.; PANNALA, A.; YANG, M.R.C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free. Radical. Biol. Med., v. 26, p. 1231–1237, 1999.
  • [16] SARMADI, B.H.; ISMAIL, A. Antioxidative peptides from food proteins: a review. Peptides, v. 53, p. 1949–56, 2010.
  • [17] TAVARES, A.P.M.; RODRÍGUEZ, O.; FERNÁNDEZ-FERNÁNDEZ, M.; DOMÍNGUEZ, A.; MOLDES, D.; SANROMÁN, M.; MACEDO, E. Immobilization of laccase on modified silica: stabilization, thermal inactivation and kinetic behaviour in 1-ethyl-3-methylimidazolium ethylsulfate ionic liquid. Bioresour. Technol., v. 131, p.405–12, 2013.
Como citar:

DUARTE NETO, J. M. W. ; MACIEL, J. C.; CARVALHO JR, L. B.; LIMA, C. A.; PORTO, A. L. F.; "PRODUCTION OF ANTIOXIDANT HYDROLYSATE FROM BOVINE CASEIN USING IMMOBILIZED PENICILLIUM AURANTIOGRISEUM PROTEASE ON MAGNETIC NANOPARTICLES", p. 3149-3155 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0182-26654-174176

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações