Full Article - Open Access.

Idioma principal

Using Scanning Electrochemical Microscopy (SECM) for measuring electrochemical current change in fibroblast cell culture treated

Zanluchi, J. M.; Pizzol, C. Dal; Stuart, R. M.; Baptista, M. C.; Marangoni, P. R. D.; Berton, M. A. C.; Ferracin, L. C.; Dieamant, G. C.;

Full Article:

Scanning electrochemical microscopy (SECM) is a technique of interest to obtain information about chemical, electrochemical, kinetics and topographical activities of materials. Even though this technique is applied to non-biological systems, as in studies of metal corrosion, recently it has been enhanced, being widely used in research labs using cell culture. In general, this technique analyzes and tracks the electric current using ultra/microelectrodes placed near the surface of a sample submerged in electrolyte solution. The action of electrical signals has extensive relationship with the human body, its physiological effects are based on the consequent improvement of nutrition, oxygenation and cells metabolism as a whole, and these signals also stimulate fibroblasts to produce more and better collagen. Therefore, this study was based on in vitro monitoring of electrochemical current in fibroblasts cell culture before and after use of cosmetic formulations containing ingredients for anti-aging treatment. Electrochemical current was evaluated in cell culture of fibroblasts by scanning cells surface and using chronoamperometry technique to measure the electrochemical current, using three different cosmetic formulations treatment: tonic, gel cream and serum, applied separately and also in combined use, in order to verify the change of bioelectricity.

Full Article:

Palavras-chave: Scanning electrochemical microscopy, electrochemical current, bioelectricity,

Palavras-chave: ,

DOI: 10.5151/chempro-s3ie2016-14

Referências bibliográficas
  • [1] JEONG, Y. Introduction to Bioelectricity. Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology. Republic of Korea, 201
  • [2] LIMA, A.S.; Uso da microscopia eletroquímica no estudo da dinâmica de processos eletródicos em superfícies funcionalizadas. Dissertação- (mestrado em Química) – Instituto de Química da Universidade de São Paulo – IQ/USP. São Paulo, 2011.
  • [3] LOOF. A.; HAES.W.; BOERJAN. B.; SCHOOFS. L.; The Fading in Electricity Theory of Ageing: The missing biophysical principle? Ageing Research Reviews. Journal homepage. Department of Biology, KU Leuven – University of Leuven, Belgium, 2012.
  • [4] MARANGONI, P.F.D.; BERTON, M.A.C.; FERRACIN, L.C.; Relatório de dados técnicos de Bioletricidade: Fase 2. Instituto SENAI de Inovação em eletroquímica. SENAI - Campus da Indústria. Curitiba Paraná, Brasil, 2015.
  • [5] NUCCITELLI, R.; NUCCITELLI, P.; LI, C.; NARSING, S.; PARISER, D.M.; LUI, KAYING. The electric field near human skin wounds declines with age and provides a non-invasive indicator of wound healing. Wound Repair Regeneration. United States, Vol. 19, p. 645-655, 2011.
  • [6] NUCCITELLI, R.; LUI, K.; TRAN, K.; ATHOS, B.; KREIS, M.; NUCCITELLI, P. Measuring the electric field in skin to detect malignant lesions. Biosensors and Molecular Technologies for Cancer Diagnostics. Editors, Keith E. Herold; Avraham Rasooly. p.765, (series in sensors), United States, 2012.
  • [7] OLIVEIRA. V.C.; A Eletroestimulação por Micros correntes na Revitalização Facial. Trabalho de Conclusão de Curso de Especialização Lato Sensu em Estética. Faculdade Redentor. Instituto Itesa. São Paulo, 2011.
  • [8] TROLLINGER, D. R.; ISSEROFF, R. R.; NUCCITELLI, R.; Calcium Channel Blockers Inhibit Galvanotaxis in Humans Keratinocytes. Journal of Cellular Physiology. Department of Dermatology, University of California, Davis, California, 2002.
Como citar:

Zanluchi, J. M.; Pizzol, C. Dal; Stuart, R. M.; Baptista, M. C.; Marangoni, P. R. D.; Berton, M. A. C.; Ferracin, L. C.; Dieamant, G. C.; "Using Scanning Electrochemical Microscopy (SECM) for measuring electrochemical current change in fibroblast cell culture treated", p. 163-167 . In: Proceedings of 2nd International Seminar on Industrial Innovation in Electrochemistry . São Paulo: Blucher, 2016. São Paulo: Blucher, 2016.
ISSN 2318-4043, DOI 10.5151/chempro-s3ie2016-14

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações