Janeiro 2015 vol. 1 num. 3 - International Symposium on Crystallography

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STRUCTURAL CHARACTERIZATION OF A RECOMBINANT TN ANTIGEN-BINDING LECTIN FROM VATAIREA MACROCARPA

Sousa, B. L. ; Silva Filho, J. C. ; Pereira, R. I. ; Rocha, B. A. M. ; Delatorre, P. ; Bezerra, G. A. ; Nagano, C. S. ; Gruber, K. ; Cavada, B.S. ;

Abstract:

Legume lectins represent the largest and most thoroughly studied lectin family, comprising a large group of homologous carbohydrate-binding proteins (Sharon Andamp; Lis, 1990). A few galactose/N-acetylgalactosamine (Gal/GalNAc) binding lectins isolated from legume plants have proven to be useful as markers in cancer histochemistry. Structural analysis of Tn-binding lectins is of considerable interest for elucidating the mechanism of specific protein-carbohydrate recognition as well as for engineering novel binding activities in legume lectins. Here we present the crystal structures of a single-chain recombinant legume lectin from Vatairea macrocarpa (VML), recombinatly expressed in Escherichia coli, in complex with lactose, N-acetyl-galactosamine and Tn antigen, respectively. The structural data is supported by isothermal titration calorimetry (ITC) and small-angle X-ray scattering assays. Molecular docking simulations were also performed to analyze the binding of this lectin to O-mucins. These findings provide a complete structural characterization of this new Tn-binding protein and strongly suggest recombinant VML as a new tool for cancer research.

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DOI: 10.5151/phypro-sic100-050

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Como citar:

Sousa, B. L.; Silva Filho, J. C.; Pereira, R. I.; Rocha, B. A. M.; Delatorre, P.; Bezerra, G. A.; Nagano, C. S.; Gruber, K.; Cavada, B.S.; "STRUCTURAL CHARACTERIZATION OF A RECOMBINANT TN ANTIGEN-BINDING LECTIN FROM VATAIREA MACROCARPA", p. 50 . In: Proceedings of the International Symposium on Crystallography [Blucher Physics Proceedings, v.1, n.3]. São Paulo: Blucher, 2015.
ISSN 2358-2359, DOI 10.5151/phypro-sic100-050

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