Production of Recombinant Enzymatic Mixture by Co-culture Strategy

Production of Recombinant Enzymatic Mixture by Co-culture Strategy

SILVA, M. R.; GOMES, D. S.; FREITAS, S.

Artigo:

Sugarcane bagasse is considered to be a residue with great potential as feedstock for second-generation ethanol production, and other added-value products. However, certain obstacles must be overcome to make this technology viable, as in the case of enzymatic route in which the high cost of enzymes production, and the low efficiency in the saccharification step still represent a bottleneck. Recently, recombinant enzymes have been used as supplement fungal cocktails to improve lignocellulosic biomass saccharification, as well as to assembly specific enzymatic mixtures aiming at other applications. In this study, we present the development of recombinant enzymatic mixture production process based on co-culture strategy. The process development includes the inoculum assembly, screening of alternative carbon sources, and improve of induction phase. Escherichia coli clones co-culture present as main advantages: the possibility to produce an enzymatic mixture with a desired composition based on inoculum assembling, the use of low cost carbon source as hemicellulosic hydrolysates based on complete metabolic machinery of E. coli, and reduction of the production process and recovery costs since more than one enzyme is produced in the same reactor.

Sugarcane bagasse is considered to be a residue with great potential as feedstock for second-generation ethanol production, and other added-value products. However, certain obstacles must be overcome to make this technology viable, as in the case of enzymatic route in which the high cost of enzymes production, and the low efficiency in the saccharification step still represent a bottleneck. Recently, recombinant enzymes have been used as supplement fungal cocktails to improve lignocellulosic biomass saccharification, as well as to assembly specific enzymatic mixtures aiming at other applications. In this study, we present the development of recombinant enzymatic mixture production process based on co-culture strategy. The process development includes the inoculum assembly, screening of alternative carbon sources, and improve of induction phase. Escherichia coli clones co-culture present as main advantages: the possibility to produce an enzymatic mixture with a desired composition based on inoculum assembling, the use of low cost carbon source as hemicellulosic hydrolysates based on complete metabolic machinery of E. coli, and reduction of the production process and recovery costs since more than one enzyme is produced in the same reactor.

Palavras-chave:

DOI: 10.5151/chemeng-cobeq2014-0799-23741-175193

Referências bibliográficas

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Como citar:

SILVA, M. R.; GOMES, D. S.; FREITAS, S.; "Production of Recombinant Enzymatic Mixture by Co-culture Strategy", p-1193-1200. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-0799-23741-175193