Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

Artigo - Open Access.

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In this work, it is shown that aqueous two-phase systems can be formed by the combination of acetonitrile and polysaccharides, namely dextran biopolymers. The respective ternary phase diagrams were determined at 25 ºC for the systems composed of water + acetonitrile + dextran. The effect of the dextran molecular weight was also ascertained toward their ability to undergo liquid-liquid demixing. An increase in the dextran molecular weight favors the phase separation. In general, acetonitrile is enriched in the top phase while dextran is majorly concentrated in the bottom phase. The applicability of this new type of two-phase systems as liquid-liquid extraction approaches was also evaluated by the study of the partition behavior of a well-known antioxidant, namely vanillin, and used here as a model biomolecule. The optimized conditions led to a recovery of vanillin of 70.7% at the acetonitrile-rich phase.



DOI: 10.5151/chemeng-cobeq2014-1782-17588-149432

Referências bibliográficas
  • [1] ALBERTSSON, P. A. Partition of cell particles and macromolecules. New York: Wiley; 1986.
  • [2] AMAN, A.; SIDDIQUI, N.N.; QADER, S.A.U. Characterization and potential applications of high molecular weight dextran produced by Leuconostoc mesenteroides AA1. Carbohydrate Polymeres. v. 87, p. 910-915, 201
  • [3] CARDOSO, G.B., MOURÃO, T., PEREIRA, F.M., FRICKS, A.T., FREIRE, M.G., SOARES, C.M.F., LIMA, A.S.. Aqueous two-phase systems based on acetonitrile and carbohydrates and their application to the extraction of vanillin. Sep. Purif. Technol. v. 104, p. 106-113, 201
  • [4] CARDOSO, G.B., SOUZA, I.N., MOURÃO, T., FREIRE, M.G., SOARES, C.M.F., LIMA, A.S., 201 Novel aqueous two-phase systems composed of acetonitrile and polyols: phase diagrams and extractive performance. Sep. Purif. Technol. In press, 201
  • [5] DAHMOLE, P. B., MAHAJAN, P., FENG, H. Phase separation conditions for sugaring-out in acetonitrile water systems. J. Chem. Eng., v. 55, p. 3803–3806, 2010.
  • [6] DONG, Z.; GU, F.; XU, F.; WANG, Q. Comparasion of four kinds of extraction techniques and kinetics of microwave-assisted extraction of vanillin from Vanilla plantifolia. Food Chemistry. v. 149, p. 54-61, 2014.
  • [7] FREIRE, M.G., LOUROS, C.L.S., REBELO, L.P.N., COUTINHO, J.A.P.. Aqueous biphasic systems composed of a water-stable ionic liquid + carbohydrates and their applications. Green Chem. v. 13, p. 1536-1545, 2011.
  • [8] MANDAL, M.K., SANT, S.B., BHATTACHARYA, P.K.. Dehydration of aqueous acetonitrile solution by pervaporation using PVA-iron oxide nanocomposite membrane. Colloid Surface A. v. 373, p. 11-21, 2011.
  • [9] POLLAK, P., ROMEDER, G., HAGEDORN, F., GELBKE, H.P.. Ullmann’s encyclopedia of industrial chemistry. 6ª Ed. Wiley Online Library, New York, 2000.
  • [10] RAO, S.R.; RAVISHANKAR, G.A. Vanilla flavor: production by conventional and biotechnological routes. Journal of Science and Food Agriculture. v. 80, p. 289-304, 2000.
  • [11] REIS, I. A. O.; SANTOS, S. B.; SANTOS, L. A.; OLIVEIRA, N.; FREIRE, M. G.; PEREIRA. J. F. B.; VENTURA, S. P. M.; COUTINHO, J. A. P.; SOARES, C. M. F.; LIMA, A. S. Recovery of Added-Value Compounds from Food Wastes: Selective Partitioning using Alcohol-Salt Aqueous Two-Phase Systems. Food Chemistry, v. 135, p. 2453 – 2461, 2012.
  • [12] Área temática: Processos Biotecnológicos 7SARUBBO, L. A., CAMPOS-TAKAKI, G. M., PORTO, A. L. F., TAMBOURGI, E. B., OLIVEIRA, L. A. A goma do cajueiro (Anacardiumoccidentale L.) como sistema inovador de extração líquido-líquido. Exacta, São Paulo, v. 5, p. 145-154, 2007.
  • [13] SIDDIQUI, N.N.; AMAN, A.; SILIPO, A.; QADER, S.A.U. Structural analysis and characterization of dextran produced by wild and mutante strains of Leuconostoc mesenteroides. Carbohydrate Polymers. v. 99, p. 331-338, 2014.
  • [14] SILVA, L. H. M., LOH, W. Sistemas aquoso bifásicos: Fundamentos e aplicações para partição/purificação de proteínas. Química Nova, v. 29, p. 1345-1351, 2006.
  • [15] TAHA, M., TENG, H.L., LEE, M.J.. Phase diagrams of acetonitrile or (acetone + water + EPPS) buffer phase separation systems at 298.15 K and quantum chemical modeling, J. Chem. Thermodyn. v. 54, p. 134-141, 2012.
  • [16] TAKAMUKU, T., TABATA, M., YAMAGUCHI, A., NISHIMOTO, J., KUMAMOTO, M., WAKITA, H., YAMAGUCHI, T.. Liquid structure of acetonitrile-water by X-ray diffraction and infrared spectroscopy, J. Phys. Chem. B. v. 102, p. 8880-8888, 1988.
  • [17] TUBIO, G.; NERLI, B.; PICÓ, G. Relationship between the protein surface hydrophobicity and its partitioning behaviour in aqueous two-phase systems of polyethyleneglycol-dextran. Journal of Chromatography B. v. 799, p. 293-301, 2004.
  • [18] VETTORI, M.H.P.B.; FRANCHETTI, S.M.M.; Continero, J. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextrasucrase. Carbohydrate Polymers. v. 88: p. 1440-1444, 2012.
  • [19] ZHANG, D., ZHANG, Y., WEN, Y., HOU, K., ZHAO, J.. Intrinsic kinetics for the synthesis of acetonitile from ethanol and ammonia over Co–Ni/c-Al2O3 catalyst. Chem. Eng. Res. Des. v. 89, p. 2147-2152, 2011.
  • [20] ZIDI, C.; TAYEB, R.; BOUKHILI, N.; DHAHBI, M. A supported liquid membrane system for efficient extraction of vanillin from aqueous solutions. Separation and Purification Technology. v. 82: p. 36-42, 2011.
  • [21] WANG, B., EZEJIAS T., FENG, H., BLASCHEK, H. Sugaring-out: A novel phase separation on extraction system. Chem. Eng. Sci, v. 63, p. 25958-2600, 2008.
  • [22] WALTON, N.J., MAYER, M.J., NARBAD, A.. Vanillin. Phytochemistry. v. 63, p. 505-515, 2003.
  • [23] WANG, B.; FENG, H.; EZEJI, T.; BLANSCHEK, H. Sugaring-out: a novel phase separation and extraction system. Chemical Engineering Science. v. 63, p. 2595-2600, 2008.
Como citar:

SOUZA, I. N.; CARDOSO, G. B.; FREIRE, M. G.; SOARES, C. M. F.; LIMA, A. S.; "NOVEL AQUEOUS TWO-PHASE SYSTEMS FORMED BY BIOCOMPATIBLE AND BIODEGRADABLE POLYSACCHARIDES AND ACETONITRILE", p. 2524-2531 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-1782-17588-149432

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