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Mycelium-based bio-composites can propose a renewable and biodegradablealternative for architectural construction materials. These biomaterials resultfrom growth of mycelium, fibrous root systems of fungi, on organic substrates incontrolled environmental conditions. This paper presents a material study thatexplores how substrate type and added supplements used for cultivating myceliumaffect the compressive strength of mycelium-based composites for use as masonryunits in architectural construction. For this purpose, samples grown usingPleurotus Ostreatus (Gray Oyster mushroom strain) on three different substrates(sawdust, straw and a mixture of sawdust and straw) with and withoutsupplementation are tested for compressive strength.