Fructooligosaccharides production in aqueous system using commercial and home-made inulinase after treatment in pressurized fluids

Fructooligosaccharides production in aqueous system using commercial and home-made inulinase after treatment in pressurized fluids

SILVA, M. F.; RIGO, D.; MOSSI, V.; GOLUNSKI, S.; KUHN, G. O.; MARCON, N. S.; DALLAGO, R.; MAZZUTTI, M. A.; TRES, M. V.; OLIVEIRA, J. V.; OLIVEIRA, D.; TREICHEL, H.

Artigo:

Fructoligosaccharides (FOS) are food additives with several health benefits, especially in the digestive tube, where they act as prebiotics presenting several advantages over other oligosaccharides synthesized industrially. This work is focused on the synthesis of fructooligosaccharides (FOS) from sucrose and inulin using free, immobilized and pre-treated inulinases from Kluyveromyces marxianus NRRL Y 7571 and Aspergillus niger in aqueous system. Initially, the influence of the different gases (propane, n-butane and LPG) on FOS production was investigated. The best results were achieved when both enzymes were previously treated with LPG. For the synthesis of FOS using inulinases from Aspergillus niger, 56.98% of GF2 and 8.47% of GF4, was obtained using inulin as substrate. Using inulinases from Kluyveromyces marxianus NRRL Y 7571, 4.8% of GF2, 5.47% of GF3 and 4.27% of GF4 was achieved, also using inulin as substrate. 1. INTRODUCTION Inulinases can be obtained from yeasts, fungus, bacteria and plants (Manzoni and Cavazzoni 1992). Special attention is given to the enzymes produced by Kluyveromyces Área temática: Engenharia e Tecnologia de Alimentos 1marxianus and Aspergillus niger, that presents promising activity on the substrate sucrose and inulin (Linde et al. 2012). These enzymes can be used in hydrolysis processes of inulin aiming at obtaining fructose syrup and fructooligosaccharides, and also in the oligosaccharides synthesis from sucrose (Risso

Fructoligosaccharides (FOS) are food additives with several health benefits, especially in the digestive tube, where they act as prebiotics presenting several advantages over other oligosaccharides synthesized industrially. This work is focused on the synthesis of fructooligosaccharides (FOS) from sucrose and inulin using free, immobilized and pre-treated inulinases from Kluyveromyces marxianus NRRL Y 7571 and Aspergillus niger in aqueous system. Initially, the influence of the different gases (propane, n-butane and LPG) on FOS production was investigated. The best results were achieved when both enzymes were previously treated with LPG. For the synthesis of FOS using inulinases from Aspergillus niger, 56.98% of GF2 and 8.47% of GF4, was obtained using inulin as substrate. Using inulinases from Kluyveromyces marxianus NRRL Y 7571, 4.8% of GF2, 5.47% of GF3 and 4.27% of GF4 was achieved, also using inulin as substrate. 1. INTRODUCTION Inulinases can be obtained from yeasts, fungus, bacteria and plants (Manzoni and Cavazzoni 1992). Special attention is given to the enzymes produced by Kluyveromyces Área temática: Engenharia e Tecnologia de Alimentos 1marxianus and Aspergillus niger, that presents promising activity on the substrate sucrose and inulin (Linde et al. 2012). These enzymes can be used in hydrolysis processes of inulin aiming at obtaining fructose syrup and fructooligosaccharides, and also in the oligosaccharides synthesis from sucrose (Risso

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DOI: 10.5151/chemeng-cobeq2014-1143-20783-145883-fi

Referências bibliográficas

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Como citar:

SILVA, M. F.; RIGO, D.; MOSSI, V.; GOLUNSKI, S.; KUHN, G. O.; MARCON, N. S.; DALLAGO, R.; MAZZUTTI, M. A.; TRES, M. V.; OLIVEIRA, J. V.; OLIVEIRA, D.; TREICHEL, H.; "Fructooligosaccharides production in aqueous system using commercial and home-made inulinase after treatment in pressurized fluids", p-4342-4349. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-1143-20783-145883-fi