Blucher Chemical Engineering Proceedings

Fevereiro 2015, vol.1, num.2 - XX Congresso Brasileiro de Engenharia Química
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FABRICATION, CHARACTERIZATION, AND FUNCTIONALIZATION OF GOLD ELECTRODES WITH POLY(ETHYLENE GLYCOL) FOR BIOSENSING APPLICATIONS

FABRICATION, CHARACTERIZATION, AND FUNCTIONALIZATION OF GOLD ELECTRODES WITH POLY(ETHYLENE GLYCOL) FOR BIOSENSING APPLICATIONS

CRULHAS, B. P.; CASTRO, C. M.; PEDROSA, V. A.

Artigo:

Enzyme-based electrodes are one of the most studied and are applied widely for metabolites detection, like glucose and lactate, byproducts in a variety of diseases, as diabetes, obesity and cancer. The electrodes miniaturization was aided by adaption of semiconductor fabrication processes. But immobilizing distinct biorecognition elements on an electrode array still a challenging process. For this, in our work we developed a multianalyte gold electrode conjugated with Poly(ethylene glycol) (PEG), a widespread polymer used in biomaterials and biotechnology, which provides an excellent matrix for enzymes, and has unique properties, as biocompatible, nontoxic and hydrophilic, creating a robust sensor for biosensing applications. The characterization and functionalization were made by electrochemical assays, using cyclic voltammetry and impedance espectroscopy and the results showed an improvement of the electrode measurements when used the PEG conjugated with ferrocene and enzymes.

Enzyme-based electrodes are one of the most studied and are applied widely for metabolites detection, like glucose and lactate, byproducts in a variety of diseases, as diabetes, obesity and cancer. The electrodes miniaturization was aided by adaption of semiconductor fabrication processes. But immobilizing distinct biorecognition elements on an electrode array still a challenging process. For this, in our work we developed a multianalyte gold electrode conjugated with Poly(ethylene glycol) (PEG), a widespread polymer used in biomaterials and biotechnology, which provides an excellent matrix for enzymes, and has unique properties, as biocompatible, nontoxic and hydrophilic, creating a robust sensor for biosensing applications. The characterization and functionalization were made by electrochemical assays, using cyclic voltammetry and impedance espectroscopy and the results showed an improvement of the electrode measurements when used the PEG conjugated with ferrocene and enzymes.

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DOI: 10.5151/chemeng-cobeq2014-1012-21665-160517

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Como citar:

CRULHAS, B. P.; CASTRO, C. M.; PEDROSA, V. A.; "FABRICATION, CHARACTERIZATION, AND FUNCTIONALIZATION OF GOLD ELECTRODES WITH POLY(ETHYLENE GLYCOL) FOR BIOSENSING APPLICATIONS", p-1433-1441. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-1012-21665-160517

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