Evaluation of lipases from metagenomic in kinetic resolution of secondary alcohols

Evaluation of lipases from metagenomic in kinetic resolution of secondary alcohols

Costa, Allen C. S.; Fraiz, Talita C.; Alnoch, Robson; Madalozzo, Aline D.; Oliveira, Alfredo R. M. de; Krieger, Nadia; Piovan, Leandro

Abstract:

Conventional techniques of enzymes relies on isolation and cultivation of microorganism. Since it is estimated that only 1-10% of microorganisms are culturable different approaches have been used for obtaining new enzymes of biotechnological interest. Metagenomic technique obviate this need by directly extracting of DNA fragments from environment and subsequent sequencing, analysis and overexpression into a host microorganism culturable in the laboratory. Since lipases obtained by metagenomic have not been evaluated for their applicability in organic synthesis, we decided to evaluate the lipases LipC6G9 and LipC12, wich were obtained from a metagenomic library constructed from a sample of animal fat contaminated soil, in the kinetic resolution of secondary alcohols and respective esters 1a-1d.

Conventional techniques of enzymes relies on isolation and cultivation of microorganism. Since it is estimated that only 1-10% of microorganisms are culturable different approaches have been used for obtaining new enzymes of biotechnological interest. Metagenomic technique obviate this need by directly extracting of DNA fragments from environment and subsequent sequencing, analysis and overexpression into a host microorganism culturable in the laboratory. Since lipases obtained by metagenomic have not been evaluated for their applicability in organic synthesis, we decided to evaluate the lipases LipC6G9 and LipC12, wich were obtained from a metagenomic library constructed from a sample of animal fat contaminated soil, in the kinetic resolution of secondary alcohols and respective esters 1a-1d.

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DOI: 10.5151/chempro-15bmos-BMOS2013_2013914223725

Referências bibliográficas

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• [3] 3 Chen, C. Fujimoto, Y. Girdaukas, G. and Sih, J. J. Am. Chem. Soc.. 1982, 104, 7294.

Como citar:

Costa, Allen C. S.; Fraiz, Talita C.; Alnoch, Robson; Madalozzo, Aline D.; Oliveira, Alfredo R. M. de; Krieger, Nadia; Piovan, Leandro; "Evaluation of lipases from metagenomic in kinetic resolution of secondary alcohols", p-250-250. In: In Blucher Chemistry Proceedings, São Paulo, v. 1, n. 2, Dezembro.2013. São Paulo: Blucher, 2013.
ISSN 23184043, DOI 10.5151/chempro-15bmos-BMOS2013_2013914223725