Development of Methodology for Glycopeptide Enrichment

Development of Methodology for Glycopeptide Enrichment

Caixeta; Vanessa; Westerlind; Ulrika

Abstract:

Glycosylation is one of the most common posttranslational modifications, found on more than 50% of proteins. The majority of glycoproteins are involved in protein-protein interactions on the extra cellular surface that influence growth factors; cellular communication; hormone activities; immunological evasion and tumor progression as examples. Due to its importance, glycoproteins are a potential source for biomarker discovery in order to detect diseases at early stage and to increase patience surveillance. It is known that disease progression is often related to pathway dysregulation including changes of the glycosylation extension as well as the carbohydrate structure. Nowadays, the strategy adopted for glycoproteins studies comprises enrichment and standard proteomic analysis using mass spectrometry (MS). However using this approach, it is obtained a small quantity of peptides containing the glyco motif compared to the total amount of peptides which makes the glycopeptides characterization difficult. For this reason it is important to propose methodologies to enrich glycopeptides or glycoproteins from others.

Glycosylation is one of the most common posttranslational modifications, found on more than 50% of proteins. The majority of glycoproteins are involved in protein-protein interactions on the extra cellular surface that influence growth factors; cellular communication; hormone activities; immunological evasion and tumor progression as examples. Due to its importance, glycoproteins are a potential source for biomarker discovery in order to detect diseases at early stage and to increase patience surveillance. It is known that disease progression is often related to pathway dysregulation including changes of the glycosylation extension as well as the carbohydrate structure. Nowadays, the strategy adopted for glycoproteins studies comprises enrichment and standard proteomic analysis using mass spectrometry (MS). However using this approach, it is obtained a small quantity of peptides containing the glyco motif compared to the total amount of peptides which makes the glycopeptides characterization difficult. For this reason it is important to propose methodologies to enrich glycopeptides or glycoproteins from others.

Palavras-chave:

DOI: 10.5151/chempro-15bmos-BMOS2013_20138512588

Referências bibliográficas

• [1] 1 Buskas, T.; Ingale, S.; Boons, G.-J. Glycobiology 2006, 16, 113.
• [2] 2 Lai, Z. W.; Nice, E. C.; Schilling O. Proteomic 2013, 13, 512.
• [3] 3 a) Sletten, E.M.; Bertozzi, C.R. Angew. Chem. Int. Ed. 2009, 48, 6974; b) Nilsson, J.; Rüetschi, U.; Halim, A.; Hesse, C.; Carlsohn, E.; Brinkmalm, G.; Larson, G. Nature Methods, 2009, 6, 809.

Como citar:

Caixeta, ; Vanessa, ; Westerlind, ; Ulrika, ; "Development of Methodology for Glycopeptide Enrichment", p-8-8. In: In Blucher Chemistry Proceedings, São Paulo, v. 1, n. 2, Dezembro.2013. São Paulo: Blucher, 2013.
ISSN 23184043, DOI 10.5151/chempro-15bmos-BMOS2013_20138512588