Detection of Canine Distemper Virus by Surface Plasmon Resonance

Detection of Canine Distemper Virus by Surface Plasmon Resonance

BASSO, C. R.; SEMPIONATTO, J. R.; TOZATO, C. C.; ARAUJO JR, J. P.; PEREZ, G. G.

Artigo:

A PMMA polymer adsorbed in gold sensor through surface plasmon resonance (SPR) technique was developed for detection the canine distemper virus (CDV) through antibody/antigen binding. The antibodies anti-CDV were physically adsorbed on the surface of the sensor through covalent bonds with the thiol group formed by PMMA/EDC-NHS, the urine samples with the CDV virus were deposited on the sensor to be detected. The highest sensitive sensor surface for urine sample detection was the 200µg/ml of immobilized in 20µg/ml of antibodies, with 1 µg/ml of detection limit yielding 50 millidegree of SPR angle shift. After each binding experiment, the sensor surface was completely regenerated prior to the next measurement using 0.2 mol

A PMMA polymer adsorbed in gold sensor through surface plasmon resonance (SPR) technique was developed for detection the canine distemper virus (CDV) through antibody/antigen binding. The antibodies anti-CDV were physically adsorbed on the surface of the sensor through covalent bonds with the thiol group formed by PMMA/EDC-NHS, the urine samples with the CDV virus were deposited on the sensor to be detected. The highest sensitive sensor surface for urine sample detection was the 200µg/ml of immobilized in 20µg/ml of antibodies, with 1 µg/ml of detection limit yielding 50 millidegree of SPR angle shift. After each binding experiment, the sensor surface was completely regenerated prior to the next measurement using 0.2 mol

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DOI: 10.5151/chemeng-cobeq2014-1074-21218-141890

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Como citar:

BASSO, C. R.; SEMPIONATTO, J. R.; TOZATO, C. C.; ARAUJO JR, J. P.; PEREZ, G. G.; "Detection of Canine Distemper Virus by Surface Plasmon Resonance", p-1483-1490. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-1074-21218-141890