EVALUATION OF Chlorella vulgaris PRODUCTION AS FUCTION OF GLYCEROL AND CORN STEEP LIQUOR CONCENTRATION USING FACTORIAL DESIGN

EVALUATION OF Chlorella vulgaris PRODUCTION AS FUCTION OF GLYCEROL AND CORN STEEP LIQUOR CONCENTRATION USING FACTORIAL DESIGN

SILVA, P. E. C.; SOUZA, F. A. S. D. de; MARQUES, D. A. V.; PORTO, A. L. F.; BEZERRA, R. P.

Artigo:

Microalgae are potential sources of high value chemicals and bioactive compounds. To promote the cell productivity, culture conditions such as carbon and nitrogen sources, irradiance, temperature, bioreactor design have been investigated. Under mixotrophic conditions, some microalgae grows rapidly and to have a higher growth rate when compared to photoautotrophic conditions. This works reports Chlorella vulgaris production under mixotrophic conditions using glycerol and corn steep licor. The factorial design (22 plus star central) was performed with 0.5-1.5% gycerol (Cgly) and 0.5-1.5% corn steep liquor (Ccsl) using maximum cell concentration (Xm), cell productivity (PX) as response. Results from the response surface methodology were that the Xm of 1.52 g L-1 was obtained using 0.9 % of G and 1.2 % of CSL. The highest PX value was 232 mg L-1 day-1 with 1.5% of G and CSL. Therefore, these models can be useful to predict maximum cell concentration in mixotrophic

Microalgae are potential sources of high value chemicals and bioactive compounds. To promote the cell productivity, culture conditions such as carbon and nitrogen sources, irradiance, temperature, bioreactor design have been investigated. Under mixotrophic conditions, some microalgae grows rapidly and to have a higher growth rate when compared to photoautotrophic conditions. This works reports Chlorella vulgaris production under mixotrophic conditions using glycerol and corn steep licor. The factorial design (22 plus star central) was performed with 0.5-1.5% gycerol (Cgly) and 0.5-1.5% corn steep liquor (Ccsl) using maximum cell concentration (Xm), cell productivity (PX) as response. Results from the response surface methodology were that the Xm of 1.52 g L-1 was obtained using 0.9 % of G and 1.2 % of CSL. The highest PX value was 232 mg L-1 day-1 with 1.5% of G and CSL. Therefore, these models can be useful to predict maximum cell concentration in mixotrophic

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DOI: 10.5151/chemeng-cobeq2014-0587-24824-140960

Referências bibliográficas

• [1] BISCHOFF H. W.; BOLD H. C. Phycological Studies IV. Some Soil Algae from Enchanted Rock and Related Algal Species,. University of Texas Publication No. 6318, 1963, p. 95.
• [2] BOX, G.E.P.; BEHNKEN. D.W. Some new three level designs for the study of quantitative variables.Technometrics, v. 2, p.455–475, 1960.
• [3] Área temática: Processos Biotecnológicos 78 CHEN, G.Q.; CHEN, F. Growing phototrophic cells without light. Biotechnol. Lett. v.28, p.607–611, 2006.
• [4] CHENG, K.C.; REN, M.; OGDEN, K.L Statistical optimization of culture media for growth and lipid production of Chlorella protothecoides UTEX 250. Biores Biotech v.128, p.44-48, 2013.
• [5] CHOJNACKA, K.; MÁRQUEZ-ROCHA, F.J. Kinetic and Stoichiometric Relationships of the Energy and Carbon metabolism in the Culture of Microalgae. Biotech., v.3, p.21-32, 2004.
• [6] GAUTAM, K.; PAREEK,A.; SHARMA, D.K. Biochemical composition of green alga Chlorella minutíssima in mixotrophic cultures under the effect of different carbon sources- J.Biosc. Bioeng., v. 116 (5), p. 624 -627, 2013 HEREDIA-ARROYO, T.; WEI, W.; HU. B. Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides Appl. Biochem. Biotechnol., v.162, p. 1978–199, 2010.
• [7] ISLETEN-HOSOGLU, M., GULTEPE, I., ELIBOL, M. Optimization of carbon and nitrogen sources for biomass and lipid production by Chlorella saccharophila under heterotrophic conditions and development of Nile red fluorescence based method for quantification of its neutral lipid content. Bioch. Eng. J., v.61, p. 11–19, 2012.
• [8] KONH, W.B.; YANG, H.; CAO, Y.T.; SONG, H.; HUA, F.; XIA, C. Effect and glycerol and glucose on the enhancement of biomass, lipid and soluble carbohydrate production by Chlorella vulgaris in mixotrophic culture. Food Technol. Biotechnol. v.51, p.62-69, 2012.
• [9] LI, Z.; YUAN, H.; YANG, J.; LI. B.Optimization of the biomass production of oil algae Chlorella. minutissima UTEX2341. Bioresour. Technol., v.102, p. 9128–9134, 2011.
• [10] LIANG, Y.; SARKANY, N.; CUI, Y. Biomass and lipid productivities of Chlorella Vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions. Biotechnol. Lett. v.31, p. 1043–1049, 2009.
• [11] LIGGETT, R.W.; KOFFLER, H. Corn steep liquor in microbiology. Lafayet University. v.12 U.S.A. (1998) MILLER T.L.; CHURCHILL, B.W. Substrates for large-scale fermentations In: A.L. Demain, L.A. Solomon (Eds.), Manual of industrial microbiology and biotechnology, American Society for Microbiology, Washington, DC (1986). MITRA, D.; VAN LEEUWEN, J.; LAMSAL, B.Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris On industrial co-products. Algal Res. v.1(1), p. 40–48, (2012) O’GRADY, J.; MORGAN J.A. Heterotrophic growth and lipid production of Chlorella protothecoides on glycerol Bioprocess Biosyst. Eng., v. 34, p. 121–125, 2010.
• [12] PATIL, P.D.; GUDE, V.G.; MANNARSWAMY, A.; DENG, S.; COOKE, P.; MUNSON-MCGEE, S.; RHODES, I.; LAMMERS P.; NIRMALAKHANDAN N. Optimization of direct conversion of wet algae to biodiesel under supercritical methanol conditions. Bioresour. Technol., v.102, p. 118–122, 2011.
• [13] PEREZ-GARCIA, O.; ESCALANTE, F.M.E.; DE-BASHAN, L.E; BASHAN Y. Heterotrophic cultures of microalgae: metabolism and potential products. Water Res., v. 45, p. 11–36, 2011.
• [14] PITTMAN, J.K.; DEAN, A.P.; OSUNDEKO, O. The potential of sustainable algal biofuel production using wastewater resources Bioresour. Technol., 102, p. 17–25, 201., RAI,P.M.; NIGAM, S.; SHARMA. Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application. Biomass Bioen., v. 58, p. 251-257, 2013.
• [15] RAMANNA, L.; GULDHE,A.; , I.; BUX,The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources. Biores. Techn., In Press, available online 27 March 2014.

Como citar:

SILVA, P. E. C.; SOUZA, F. A. S. D. de; MARQUES, D. A. V.; PORTO, A. L. F.; BEZERRA, R. P.; "EVALUATION OF Chlorella vulgaris PRODUCTION AS FUCTION OF GLYCEROL AND CORN STEEP LIQUOR CONCENTRATION USING FACTORIAL DESIGN", p-873-880. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-0587-24824-140960