AGGLUTININS EXTRACTION FROM CHLORELLA VULGARIS USING THREE DIFFERENT BUFFERS

AGGLUTININS EXTRACTION FROM CHLORELLA VULGARIS USING THREE DIFFERENT BUFFERS

CAVALCANTI, V. L. R.; NASCIMENTO, C. O.; COSTA, R. M. P. B.; CARVALHO, J. C. M.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA

Artigo:

Agglutinins are carbohydrates applied as tools in cell biology, immunology, in the study and treatment of cancers, and in a range of areas of biochemical and biomedical research. This study aims to determine the best method of possible hemagglutinating substances extraction from Chlorella vulgaris biomass using three different buffers. The cultured was concentrated by a centrifugation at 4,000 xg for 10 min, lyophilized and extracted under homogenization in pure and in concentrations of 1:5 to 1:20 using the buffers 0.025M Tris-HCl-saline, pH 7.0; 0.02M saline-phosphate, pH 7.4; and 0.1M sodium acetate, pH, 5.5. In microtiter boards, biomass extracts were added to rabbit erythrocytes for hemagglutination activity. Positive results were obtained in Tris-HCl-saline buffer (1:10 and 1:20 dilutions) and sodium acetate buffer (pure extract and dilutions) with hemagglutination titers of 2

Agglutinins are carbohydrates applied as tools in cell biology, immunology, in the study and treatment of cancers, and in a range of areas of biochemical and biomedical research. This study aims to determine the best method of possible hemagglutinating substances extraction from Chlorella vulgaris biomass using three different buffers. The cultured was concentrated by a centrifugation at 4,000 xg for 10 min, lyophilized and extracted under homogenization in pure and in concentrations of 1:5 to 1:20 using the buffers 0.025M Tris-HCl-saline, pH 7.0; 0.02M saline-phosphate, pH 7.4; and 0.1M sodium acetate, pH, 5.5. In microtiter boards, biomass extracts were added to rabbit erythrocytes for hemagglutination activity. Positive results were obtained in Tris-HCl-saline buffer (1:10 and 1:20 dilutions) and sodium acetate buffer (pure extract and dilutions) with hemagglutination titers of 2

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DOI: 10.5151/chemeng-cobeq2014-0555-24971-151835

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Como citar:

CAVALCANTI, V. L. R.; NASCIMENTO, C. O.; COSTA, R. M. P. B.; CARVALHO, J. C. M.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA; "AGGLUTININS EXTRACTION FROM CHLORELLA VULGARIS USING THREE DIFFERENT BUFFERS", p-784-789. In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 23591757, DOI 10.5151/chemeng-cobeq2014-0555-24971-151835