fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

Artigo - Open Access.

Idioma principal

TWO-PHASE PARTITIONING OF A COLLAGENASE PRODUCED BY YEAST (13II) ISOLATED FROM BEE POLLEN (Melípona spp.)

ARRUDA, A. A.; LUIZLUIZ, A. C.; MOREIRA, K. A.; MARQUES, D. A. Viana; PORTO, A. L. F.; LIMA, C. A.;

Artigo:

The potential application of aqueous two-phase systems of PEG/phosphate to recovery collagenase from yeast (13II) fermentation broth was investigated. To plan the experimental tests and evaluate the corresponding

Artigo:

Palavras-chave:

DOI: 10.5151/chemeng-cobeq2014-0104-27023-137252

Referências bibliográficas
  • [1] ARAÚJO, R.F.F.; PORTO, T.S.; MARTINS, D.B.G.; DUTRA, R.F., PORTO, A.L.F., LIMA-FILHO, J.L. Partitioning of lactate dehydrogenase from bovine heart crude extract by polyethylene glycol–citrate aqueous two-phase systems. Fluid Phase Equilib., v. 301, p. 46–50, 201
  • [2] BABU, B.R.; RASTOGI, N.K.; RAGHAVARAO, K.S.M.S. Liquid–liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase syste. Chem. Eng. Process., v. 47, p. 83–89, 2008.
  • [3] CHAVIRA, R.J.; BURNETT, T.J.; HAGEMAN, J.H. Assaying proteinases with azocoll. Anal Biochem. v. 136, p. 4446–4450, 1984.
  • [4] HAMDY, H.S.. Extracellular collagenase from Rhizoctonia solani: production, purification and characterization. Indian J. Biotechnol., v. 7, p. 333-340, 2008.
  • [5] IMELIO, N.; MARINI, A.; SPELZINI, D.; PICÓ, G.; FARRUGGIA, B. Pepsin extraction from bovine stomach using aqueous two-phase systems: Molecular mechanism and Área temática: Processos Biotecnológicos 6influence of homogenate mass and phase volume ratio. J. Chromatogr. B., v. 873, p. 133–138, 2008.
  • [6] JARA, F.; PILOSOF, A.M.R. Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems. Food Hydrocolloid. v. 25, p. 374-380, 2011.
  • [7] NICKERSON, W.J.; MOHAN, R.R. Studies on the nutrition and metabolism of Streptomyces. In: Symposium on Actinomycetales, International Comgress of Microbiology, 6º Congress, Roma, p. 137-146, 1953.
  • [8] SAKURAI, Y.; INOUE, H.; NISHII, W.; TAKAHASHI, T.; LINO, Y.; YAMAMOTO, M.; TAKAHASHI, K. Purification and characterization of a major collagenase from Streptomyces parvulus. Biosci. Biotechnol. Biochem., v. 73, p. 21-28, 2009.
  • [9] SARAVANAN, S.; RAO, J.R.; NAIR, B.U.; RAMASAMI, T. Aqueous two-phase poly(ethylene glycol)–poly(acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature. Process Biochem., v. 43, p. 905–911, 2008.
  • [10] SMITH, P.K.; KROHN, R.I.; HERMANSON, G.T.; MALLIA, A.K.; GARDENER, F.H.; PREVENANO, M.D.,; FUJIMOTO, C.K.; GOEKE, N.M.; OLSON, B.J.; KLENK, D.C. Measurement of protein using bicinchoninic acid. Anal Biochem., v. 150, p. 76–85, 1985.
  • [11] TUBIO, G.; PICÓ, G.A.; NERLI, B.B. Extraction of trypsin from bovine pancreas by applying polyethyleneglycol/sodium citrate aqueous two-phase systems. J. Chromatogr. B., v. 877, p. 115–120, 2009.
  • [12] WU, Q.; LI, C.; CHEN, H.; SHULIANG, L. Purification and characterization of a novel collagenase from Bacillus pumilus Col-J. Appl. Biochem. Biotechnol., v. 160, p. 129-139, 2010.
Como citar:

ARRUDA, A. A.; LUIZLUIZ, A. C.; MOREIRA, K. A.; MARQUES, D. A. Viana; PORTO, A. L. F.; LIMA, C. A.; "TWO-PHASE PARTITIONING OF A COLLAGENASE PRODUCED BY YEAST (13II) ISOLATED FROM BEE POLLEN (Melípona spp.)", p. 128-134 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0104-27023-137252

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações