Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química
Artigo - Open Access.
GREEN ROUTE FOR AMOXICILLIN PRODUCTION THROUGH THE INTEGRATION WITH THE RECYCLE OF THE BY-PRODUCT (P-HYDROXYPHENYLGLYCINE)
In the kinetically-controlled enzymatic synthesis of amoxicillin catalyzed by penicillin G acylase (PGA, E.C.184.108.40.206) p-hydroxyphenylglycine (PHPG) is a by-product continuously formed in the reaction. Also, methyl ester is the standard substrate used in the production of amoxicillin generating methanol as a by-product. Thus, we assessed the recovery and reuse of PHPG as reactant for the synthesis of p-hydroxyphenylglycine ethyl ester substrate (PHPGEE) and the integration to the process through the recycle of PHPGEE for the reactor of enzymatic synthesis of amoxicillin generating ethanol as a by-product. Recovery of the by-product of the enzymatic synthesis of amoxicillin was effective reaching a purity of 99% for the PHPG. Purified PHPG was successfully employed in the production of PHPGEE with a conversion of 93%. The enzymatic synthesis of amoxicillin employing the previously synthesized PHPGEE was feasible following the characteristic profile that is expected for these reactions.
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-  6. ACKNOWLEDGEMENTS The authors would like to thank Brazilian research-funding agencies (CAPES, CNPq and FAPESP) and the group of the Prof. Guisán (Department of Enzymatic Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain) for the biocatalyst.
PEREIRA, S. C.; CASTRAL, T. C.; RIBEIRO, M. P. A.; GIORDANO, R. L. C.; GIORDANO, R. C.; "GREEN ROUTE FOR AMOXICILLIN PRODUCTION THROUGH THE INTEGRATION WITH THE RECYCLE OF THE BY-PRODUCT (P-HYDROXYPHENYLGLYCINE)", p. 1823-1830 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2].
São Paulo: Blucher,
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-1263-20189-138533
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