Fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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Compressed N-Butane for treatment of commercial and non-commercial inulinases

SILVA, M. F. ; RIGO, D. ; MOSSI, V. ; GOLUNSKI, S. ; DILUCCIO, M. ; MAZZUTTI, M. A. ; TRES, M. V. ; OLIVEIRA, J. V. ; OLIVEIRA, D. ; TREICHEL, H. ;

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Inulinase from Klyveromyces marxianus NRRL Y-7571 presented an increase of 210 % in the residual activity using n-butane at 30 bar during 6 h exposure and with a depressurization rate of 20 bar/min. For commercial inulinase from Aspergillus niger, an increment of 142 % in the residual activity at 270 bar for 6 h treatment at the highest depressurization rate (100 bar/min) was observed. The use of compressed n-butane may be of technological relevance as a preceding, preparation step, towards improving enzyme activity, hence helping the development of new biotransformation processes. 1. INTRODUCTION Inulinases are enzymes potentially useful in the production of high fructose syrups (HFS) by enzymatic hydrolysis of inulin, affording a yield as high as 95 %. These enzymes are widely used for the production of fructooligosaccharides, compounds with functional and nutritional properties for use in low-calorie diets, stimulation of Bifidus and as a source of dietary fiber in food preparations (Ettalibi et al., 2001) To date, the high cost of enzyme production has been probably one of the major obstacles to commercialization of enzyme-catalyzed processes. For this reason, recent advances in enzyme Área temática: Engenharia e Tecnologia de Alimentos 1technology, such as the use of solvent-tolerant and/or immobilized inulinases, which make possible the re-utilization of the catalyst, have been made to develop cost-effective systems (Riss

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DOI: 10.5151/chemeng-cobeq2014-1150-20762-145874-fi

Referências bibliográficas
  • [1] ANDRADE J.M., OESTREICHER E.G., ANTUNES O.A.C., OLIVEIRA J.V., OLIVEIRA D., SOARES C.M., DARIVA C.; Effect of treatment with compressed CO2 and propane on D-hydantoinase activity. J Supercritical Fluids, 2008: v.46: 342-350.
  • [2] CELIA E., CERNIA E., PALOCCI C., SORO S., TURCHET T., Tunning Pseudomonas cepacea lipase (PCL) activity in supercritical fluids. J. of Supercritical Fluids, 2005: v.33: 193.
  • [3] ETTALIBI M., BARATTI J.C.; Sucrose hydrolysis by thermostable immobilized inulinases from Aspergillus ficuum. Enzyme Microb. Tech., 2001: v.28: 596-601.
  • [4] KNEZ Z.; Enzymatic reactions in dense gases. J. of Supercritical Fluids, 2009: v.47: 357-372.
  • [5] MAZUTTI, M.A., ZABOT, G., BONI, G., SKOVRONSKI, A., OLIVEIRA, D., DI LUCCIO, M., RODRIGUES, M.I., TREICHEL, H., MAUGERI, F. 2010. Optimization of inulinase production by solid-state fermentation in a packed-bed bioreactor, J. Chem. Technol. Biotechnol. 85, 109-114.
  • [6] RISSO, F.V.A., MAZUTTI, M.A., TREICHEL, H., COSTA, F., MAUGERI, F., RODRIGUES, M.I. 2012. Comparison between systems for synthesis of fructooligosaccharides from sucrose using free inulinase from Kluyveromyces marxianus NRRL Y-7571. Food Bioproc. Technol. 5, 331-337.
  • [7] SILVA, M.F., GOLUNSKI, S.M., RIGO, D., MOSSI, V., DI LUCCIO, M., MAZUTTI, M.A., OLIVEIRA, D., OLIVEIRA, J.V., TREICHEL. H. 2012. Pressurized Propane: An Alternative Technique to Increase Inulinase Activity, Ind. Biotechnol. 8, 293-299.
  • [8] SILVA, M.F., GOLUNSKI, S.M., RIGO, D., MOSSI, V., DI LUCCIO, M., MAZUTTI, M.A., PERGHER, S.B.C., OLIVEIRA, D., OLIVEIRA, J.V., TREICHEL, H. 2013. Liquefied petroleum gas as solvent medium for the treatment of immobilized inulinases. J. Chem. Technol. Biotechnol. 91, 54-59.
  • [9] TREICHEL, H., OLIVEIRA, D., LERIN, L., ASTOLFI, V., MAZUTTI, M.A., DI LUCCIO, M., OLIVEIRA, J.V. 2012. A review on the production and partial characterization of microbial inulinases. Global J. Biochem. 3, 7-1
Como citar:

SILVA, M. F.; RIGO, D.; MOSSI, V.; GOLUNSKI, S.; DILUCCIO, M.; MAZZUTTI, M. A.; TRES, M. V.; OLIVEIRA, J. V.; OLIVEIRA, D.; TREICHEL, H.; "Compressed N-Butane for treatment of commercial and non-commercial inulinases", p. 4366-4373 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-1150-20762-145874-fi

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