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QUANTIFICATING THE OPHTHALMIC IRRITATION TEST OF PROTICIONIC LIQUIDS USING IMAGEJ AND PHOTOSKAPE

QUANTIFICAÇÃO DO TESTE DE IRRITAÇÃO OFTÁLMICA DE LÍQUIDOS IÔNICOS PRÓTICOS USANDO IMAGEJ E PHOTOSKAPE

Andrade, Rebecca da Silva ; Veloso, Gabriela Brasil Romão; ChiariAndreo, Bruna Galdorfini ; Duro, Miguel Angel Iglesias ;

Artigo completo:

The HET-CAM (Hen‟s Egg Chorioallantoic Membrane) assay is aqualitative alternative method to the in vivo Draize Rabbit Eye test to assess theirritancy potential of chemicals. In this work, for the first time the ophthalmic irritationof 13 different protic ionic liquids has been evaluated using ImageJ and PhotoSkapeimage processing programs to analyze the results of the HET-CAM assay. Theirritation potential of a substance can be quantified using these computational toolsby observing blood vessel changes like lysis, hemorrhage and coagulation. Inconclusion, the modification of the established HET-CAM assay made it possibleto determine the damage to minute blood vessels, highlighting the low-irritantprofile of some studied protic ionic liquids to the ocular tissues.

Artigo completo:

O ensaio HET-CAM (Membrana Corioalantóica do Ovo de Galinha) é um método alternativo qualitativo para o teste in vivo de Draize para avaliar o potencial de irritação de produtos químicos. Neste trabalho, pela primeira vez, a irritação oftálmica de 13 líquidos iônicos próticos (LIPs) diferentes foi avaliada usando os programas de processamento de imagem ImageJ e PhotoSkape para analisar os resultados do ensaio HET-CAM. O potencial de irritação de uma substância pode ser quantificado usando essas ferramentas computacionais, observando as alterações dos vasos sanguíneos, como lise, hemorragia e coagulação. Em conclusão, a modificação deste ensaio tornou possível determinar o dano aos vasos sanguíneos diminutos, destacando o perfil de baixa irritação dos LIPs para os tecidos oculares.

Palavras-chave: Protic ionic liquids; ocular irritation, ImageJ, HET-CAM test.,

Palavras-chave: Líquidos iônicos próticos; irritação ocular, ImageJ, teste HET-CAM,

DOI: 10.5151/siintec2020-QUANTIFICATINGTHE

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Como citar:

Andrade, Rebecca da Silva ; Veloso, Gabriela Brasil Romão; ChiariAndreo, Bruna Galdorfini ; Duro, Miguel Angel Iglesias ; "QUANTIFICATING THE OPHTHALMIC IRRITATION TEST OF PROTICIONIC LIQUIDS USING IMAGEJ AND PHOTOSKAPE", p. 208-216 . In: Anais do VI Simpósio Internacional de Inovação e Tecnologia. São Paulo: Blucher, 2020.
ISSN 2357-7592, ISBN: 2357-7592
DOI 10.5151/siintec2020-QUANTIFICATINGTHE

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