Setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

Oral - Open Access.

Idioma principal

PREPARATION AND CHARACTERIZATION OF FUNCTIONALIZED RICE HUSK SILICA FOR LIPASE ADSORPTION

MACHADO, N. B; MIGUEZ, J. P; BOLINA, I. C. A; MENDES, A. A; , ;

Oral:

Silica has been extracted from rice husks via a simple hydrothermal process and functionalized with triethoxy(octyl)silane (OCTES) and (3- aminopropyl)triethoxysilane (3-APTES) to be used as supports to immobilize lipase from Thermomyces lanuginosus (TLL) via physical adsorption. TLL immobilization on the support functionalized with OCTES (Octyl–SiO2) and 3-APTES (Amino–SiO2) occurred via hydrophobic and ionic interactions, respectively. The supports were characterized by particle size, atomic force microscopy (AFM), elemental analysis (CHN), N2 physisorption analysis and Rose Bengal dye adsorption, thus confirming their functionalization. TLL was fully adsorbed on both functionalized supports while partial adsorption on non-functionalized support has been observed. These results show that functionalized SiO2 can be successfully used as supports in lipase immobilization.

Oral:

Palavras-chave: immobilization,

Palavras-chave:

DOI: 10.5151/cobeq2018-CO.136

Referências bibliográficas
  • [1] ADLERCREUTZ P, Immobilisation and application of lipases in organic media. Chem. Soc.
  • [2] Rev., v. 42, p. 6406-6436, 2013.
  • [3] ALVES MD, ARACRI FM, CREN EC, MENDES AA, Isotherm, kinetic, mechanism and
  • [4] thermodynamic studies of adsorption of a microbial lipase on a mesoporous and hydrophobic
  • [5] resin. Chem. Eng. J., v. 311, p. 1-12, 2017.
  • [6] BRADFORD MM, Rapid and sensitive method for the quantitation of microgram quantities
  • [7] of protein utilizing the principle of protein-dye binding. Anal. Biochem., v. 72, p. 248-254,
  • [8] 1976.
  • [9] VESCOVI V, KOPP W, GUISAN JM, GIORDANO RLC, MENDES AA, TARDIOLLI PW,
  • [10] Improved catalytic properties of Candida antarctica lipase B multi-attached on tailor-made
  • [11] hydrophobic silica containing octyl and multifunctional amino-glutaraldehyde spacer arms.
  • [12] Process Biochem., v. 51, p. 2055-2066, 2016.
  • [13] ZHANG S, GAO H, LI J, HUANG Y, ALSAEDI A, HAYAT T, XU X, WANG J, Rice husks
  • [14] as a sustainable silica source for hierarchical flower-like metal silicate architectures
  • [15] assembled into ultrathin nanosheets for adsorption and catalysis. Hazard. Mater., v. 321 p. 92-
  • [16] 102, 2017.
  • [17] ZUCCA P, SANJUST E, Inorganic materials as supports for covalent enzyme
  • [18] immobilization: Methods and mechanisms. Molecules, v. 19, p. 14139-14194, 2014.
Como citar:

MACHADO, N. B; MIGUEZ, J. P; BOLINA, I. C. A; MENDES, A. A; , ; "PREPARATION AND CHARACTERIZATION OF FUNCTIONALIZED RICE HUSK SILICA FOR LIPASE ADSORPTION", p. 4614-4617 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-CO.136

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