Junho 2019 vol. 2 num. 1 - Encontro Anual da Biofísica 2019

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PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS

PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS

Riquelme, Bibiana ; Estrada, Ezequiel ; Castellini, Horacio ; Acosta, Andrea ; Chinelatto, Alejandro ; Tac, Ivan ; Borraz, Javier ; Tullio, Liliana Di ; Galassi, Mariel E. ;

Artigo completo:

INTRODUCTION Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008).

Artigo completo:

INTRODUCTION Transfusion-associated graft-versus-host disease can be prevented by treating cellular blood products with gamma irradiation. A wide range of gamma irradiation dose levels are used in routine practice, but gamma irradiation dose of 25 Gy may be required to completely inactivate T cells in Red Blood Cells (RBC) units (Pelszynski, M. et al., 1994). This process decreases the survival of the RBC transfused, so it is crucial to understand the alterations caused by gamma irradiation to the erythrocyte membrane. In previous works, the biochemical and hematological effects of gamma irradiation at different storage periods were studied. It was observed that irradiation of the erythrocytes increases red cells hemolysis and leakage of intracellular potassium (Adams, F. et al., 2015; Yousuf, R. et al., 2018). The mechanisms through which irradiation causes the loss of RBC viability could be related to the primary effects of radiation. Gamma and X-ray Ionizing radiation cause indirect damage through the reactive oxygen species generated by water radiolysis (Anand, A.J. et al., 1997). The reduced deformability of RBC after irradiation could be related to the interaction of the oxygen-derived radicals with the membranes, affecting their mechanical properties and leading to deformability impairment (Kim, Y.-K. et al., 2008).

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DOI: 10.5151/biofisica2019-30

Referências bibliográficas
  • [1] ADAMS, F. et al. Biochemical Storage Lesions Occurring in Nonirradiated and Irradiated Red lood Cells: A Brief Review. BioMed Research International, Article ID 968302, 2015.
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Como citar:

Riquelme, Bibiana; Estrada, Ezequiel; Castellini, Horacio; Acosta, Andrea; Chinelatto, Alejandro; Tac, Ivan; Borraz, Javier; Tullio, Liliana Di; Galassi, Mariel E.; "PRELIMINARY STUDY OF ALTERATIONS IN HUMAN RED BLOOD CELLS BY IRRADIATION WITH HIGH ENERGY PHOTONS", p. 107-108 . In: Anais do Encontro Anual da Biofísica 2019. São Paulo: Blucher, 2019.
ISSN 2526--607-1, DOI 10.5151/biofisica2019-30

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