setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

Pôster - Open Access.

Idioma principal

Physiology and Transcriptomic analysis of Saccharomyces cerevisiae in the presence of inhibitors derived from lignocellulosic biomass

PROCÓPIO, D. P.; CIAMPONI, F. E.; BRANDÃO, M.; WINK, F. V.; BASSO, T. O.;

Pôster:

Production of biofuels from lignocellulosic (LC) residues should be fuelingthe energy matrix in the near future. Yeasts will play an important role as platformmicroorganisms for the conversion processes of LC-derived sugars into, for instance,fuel ethanol but also other advanced fuels and chemicals. Fermentation of LChydrolysates poses many scientific and technological challenges. For example, thepretreatment processes generates various yeast growth inhibitors (furan-derivatives,phenolics and organic acids), reducing the efficiency of fermentation. Therefore, weaimed to investigate the effect of selected inhibitory compounds on the quantitativephysiology of yeasts as well as on their transcriptional responses. For that, two S.cerevisiae strains were chosen: the lab strain S. cerevisiae CEN.PK113-7D, a referencelaboratory strain for physiological studies and functional genomics, and the robustindustrial strain S. cerevisiae SA-1, a strain highly tolerant toward inhibitorycompounds and rarely studied so far. The dataset presented so far, indicates importantphysiological differences of industrial strains, such as S. cerevisiae SA-1, in relation tolaboratory ones. Omics data in the presence of inhibitors will contribute further toevaluate these differences.

Pôster:

Palavras-chave: ,

Palavras-chave: ,

DOI: 10.5151/cobeq2018-PT.0541

Referências bibliográficas
  • [1] Basso, Luiz C., Henrique V. de Amorim, Antonio J. de Oliveira, and Mario L. Lopes. 2008. “Yeast Selection for Fuel Ethanol Production in Brazil.” FEMS Yeast Research 8(7):1155–63. Retrieved September 24, 2017 (https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00428.x). Basso, Thiago Olitta, Marcelo Goulart Dario, Aldo Tonso, Boris Ugarte Stambuk, and Andreas Karoly Gombert. 2010. “Insufficient Uracil Supply in Fully Aerobic Chemostat Cultures of Saccharomyces Cerevisiae Leads to Respiro-Fermentative Metabolism and Double Nutrient-Limitation.” Biotechnology Letters 32(7):973–77. Retrieved September 26, 2017 (http://link.springer.com/10.1007/s10529-010-0248-2). Caspeta, Luis, Tania Castillo, and Jens Nielsen. 2015. “Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes.” Frontiers in Bioengineering and Biotechnology 3:184. Retrieved September 22, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/26618154). Della-Bianca, Bianca E., Erik de Hulster, Jack T. Pronk, Antonius J. A. van Maris, and Andreas K. Gombert. 2014. “Physiology of the Fuel Ethanol Strain Saccharomyces Cerevisiae PE-2 at Low pH Indicates a Context-Dependent Performance Relevant for Industrial Applications.” FEMS Yeast Research 14(8):1196– 1205. Retrieved September 26, 2017 (https://academic.oup.com/femsyr/article-lookup/doi/10.1111/1567- 1364.12217). Della-Bianca, Bianca Eli, Thiago Olitta Basso, Boris Ugarte Stambuk, Luiz Carlos Basso, and Andreas Karoly Gombert. 2013. “What Do We Know about the Yeast Strains from the Brazilian Fuel Ethanol Industry?” Applied Microbiology and Biotechnology 97(3):979–9 Retrieved September 22, 2017 (http://link.springer.com/10.1007/s00253-012-4631-x). van Dijken JP et al. 2000. “An Interlaboratory Comparison of Physiological and Genetic Properties of Four Saccharomyces Cerevisiae Strains.” Enzyme and Microbial Technology 26(9–10):706–14. Retrieved September 24, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/10862876). Illumina. 2014. “FOR RESEARCH USE ONLY TruSeq ® RNA Sample Preparation v2 Guide.” Retrieved April 14, 2018 (https://support.illumina.com/content/dam/illuminasupport/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqrna/truseq-rnasample-prep-v2-guide-15026495-f.pdf). Klinke, H. B., A. B. Thomsen, and B. K. Ahring. 2004. “Inhibition of Ethanol-Producing Yeast and Bacteria by Degradation Products Produced during Pre-Treatment of Biomass.” Applied Microbiology and Biotechnology 66(1):10–26. Retrieved September 22, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/15300416). van Maris, Antonius J. A. et al. 2006. “Alcoholic Fermentation of Carbon Sources in Biomass Hydrolysates by Saccharomyces Cerevisiae: Current Status.” Antonie van Leeuwenhoek 90(4):391–418. Retrieved September 22, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/17033882). Palmqvist, Eva and Bärbel Hahn-Hägerdal. 2000. “Fermentation of Lignocellulosic Hydrolysates. I: Inhibition and Detoxification.” Bioresource Technology. van der Pol, Edwin C., Robert R. Bakker, Peter Baets, and Gerrit Eggink. 2014. “By-Products Resulting from Lignocellulose Pretreatment and Their Inhibitory Effect on Fermentations for (Bio)chemicals and Fuels.” Applied Microbiology and Biotechnology 98(23):9579–93. Retrieved September 24, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/25370992). Simms, D., P. Cizdziel, and P. Chomczynski. 1993. “TRIzol: A New Reagent for Optimal Single-Step Isolation of RNA.” Focus 99–102. Retrieved (http://www.invitrogen.co.jp/focus/154099.pdf%5Cnpapers2://publication/uuid/B68F1A0C-FB1A-4A64- 8567-DE4CA8297D16). Skerker, Jeffrey M. et al. 2013. “Dissecting a Complex Chemical Stress: Chemogenomic Profiling of Plant Hydrolysates.” Molecular Systems Biology 9(1):674. Retrieved September 22, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/23774757). Verduyn, Cornelis, Erik Postma, W.Alexander Scheffers, and Johannes P. Van Dijken. 1992. “Effect of Benzoic Acid on Metabolic Fluxes in Yeasts: A Continuous-Culture Study on the Regulation of Respiration and Alcoholic Fermentation.” Yeast 501–17.
Como citar:

PROCÓPIO, D. P.; CIAMPONI, F. E.; BRANDÃO, M.; WINK, F. V.; BASSO, T. O.; "Physiology and Transcriptomic analysis of Saccharomyces cerevisiae in the presence of inhibitors derived from lignocellulosic biomass", p. 2044-2047 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-PT.0541

últimos 30 dias | último ano | desde a publicação


downloads


visualizações


indexações