setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química
Pôster - Open Access.
PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE
ROCHA, J. C.; ARAÚJO, J. S.; PAIVA, W. K. V.; FERREIRA, C. R. R.; SILVA, M. G. F.; SANTOS, E. S.; SOUSA JR, F. C.;
The purpose of this study was to assess pectinase production through solidstatefermentation by Aspergillus niger IOC 4003 using caja depulping residue. Enzymeproduction was carried out in 250 mL Erlenmeyer flasks containing 5 g of washed cajaresidue or unwashed caja residue supplemented with KH2PO4 (1.5 g/L) and (NH4)2SO4(3.3 g/L). Initial pH and moisture were adjusted for 4.5 and 40%, respectively. SSF wasstarted after inoculation of 1 x 107spores/g residue of A. niger and incubated at 30°C.The microorganism was assessed during 240 hours of cultivation where at every 24 hoursthe complex enzymatic were extracted with acetate buffer (pH = 4.5, 200 mM) at ratio1:10 (g residue:mL buffer). There were measured polygalacturonase, pectin lyase andtotal cellulase activity as well as quantification of total protein and total reducing sugars.The best residue was WCR where it was obtained the maximum enzymatic production ofPFase, PGase and PNLase activity of 3.49 ± 0.08 IU/g (48 h), 38.2 ± 0.63 IU/g (72 h),8.58 ± 0.78 IU/g (48 h), respectively. Initial encountered TRS was 66.0 ± 0.2 mg/g forWCR. Therefore, washed caja residue demonstrated a great potential for the productionof pectinolytic enzymes and using these enzymes in industrial applications.
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ROCHA, J. C.; ARAÚJO, J. S.; PAIVA, W. K. V.; FERREIRA, C. R. R.; SILVA, M. G. F.; SANTOS, E. S.; SOUSA JR, F. C.; "PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE", p. 2145-2148 . In: .
São Paulo: Blucher,
ISSN 2359-1757, DOI 10.5151/cobeq2018-PT.0568
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