PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE

PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE

ROCHA, J. C.; ARAÚJO, J. S.; PAIVA, W. K. V.; FERREIRA, C. R. R.; SILVA, M. G. F.; SANTOS, E. S.; SOUSA JR, F. C.

Pôster:

The purpose of this study was to assess pectinase production through solidstatefermentation by Aspergillus niger IOC 4003 using caja depulping residue. Enzymeproduction was carried out in 250 mL Erlenmeyer flasks containing 5 g of washed cajaresidue or unwashed caja residue supplemented with KH2PO4 (1.5 g/L) and (NH4)2SO4(3.3 g/L). Initial pH and moisture were adjusted for 4.5 and 40%, respectively. SSF wasstarted after inoculation of 1 x 107spores/g residue of A. niger and incubated at 30°C.The microorganism was assessed during 240 hours of cultivation where at every 24 hoursthe complex enzymatic were extracted with acetate buffer (pH = 4.5, 200 mM) at ratio1:10 (g residue:mL buffer). There were measured polygalacturonase, pectin lyase andtotal cellulase activity as well as quantification of total protein and total reducing sugars.The best residue was WCR where it was obtained the maximum enzymatic production ofPFase, PGase and PNLase activity of 3.49 ± 0.08 IU/g (48 h), 38.2 ± 0.63 IU/g (72 h),8.58 ± 0.78 IU/g (48 h), respectively. Initial encountered TRS was 66.0 ± 0.2 mg/g forWCR. Therefore, washed caja residue demonstrated a great potential for the productionof pectinolytic enzymes and using these enzymes in industrial applications. 

The purpose of this study was to assess pectinase production through solidstatefermentation by Aspergillus niger IOC 4003 using caja depulping residue. Enzymeproduction was carried out in 250 mL Erlenmeyer flasks containing 5 g of washed cajaresidue or unwashed caja residue supplemented with KH2PO4 (1.5 g/L) and (NH4)2SO4(3.3 g/L). Initial pH and moisture were adjusted for 4.5 and 40%, respectively. SSF wasstarted after inoculation of 1 x 107spores/g residue of A. niger and incubated at 30°C.The microorganism was assessed during 240 hours of cultivation where at every 24 hoursthe complex enzymatic were extracted with acetate buffer (pH = 4.5, 200 mM) at ratio1:10 (g residue:mL buffer). There were measured polygalacturonase, pectin lyase andtotal cellulase activity as well as quantification of total protein and total reducing sugars.The best residue was WCR where it was obtained the maximum enzymatic production ofPFase, PGase and PNLase activity of 3.49 ± 0.08 IU/g (48 h), 38.2 ± 0.63 IU/g (72 h),8.58 ± 0.78 IU/g (48 h), respectively. Initial encountered TRS was 66.0 ± 0.2 mg/g forWCR. Therefore, washed caja residue demonstrated a great potential for the productionof pectinolytic enzymes and using these enzymes in industrial applications. 

Palavras-chave:

DOI: 10.5151/cobeq2018-PT.0568

Referências bibliográficas

• [1] AHMED I, ZIA MA, HUSSAIN MA, AKRAM Z, NAVEED MT, Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger: its purification and characterization. J Radiat Res Appl Sci, v. 9, p. 143-154, 2016. COURI S, FARIAS AX, Genetic manipulation of Aspergillus niger for increased synthesis of pectinolytic enzymes. Rev Microbiol, v. 26, p. 314—7, 1995. GHOSE T, Measurement of cellulase actitivities. Internat Uni Pure Appl Chem, v. 59, p. 257- 268, 1987. PITOL LO, BIZ A, MALLMANN E, KRIEGER N, MITCHELL DA, Production of pectinases by solid-state fermentation in a pilot-scale packed-bed bioreactor. Chem Engin J, v. 283, p. 1009-1018, 2016. Available on http://dx.doi.org/10.1016/j.cej.2015.08.046. SANTOS SFM, MACEDO GR, SILVA FLH, SOUZA RLA, PINTO GAS, Aplicação da metodologia de superfície de resposta no estudo da produção e extração da poligalacturonases. Quí. Nova, v. 31, p. 1973-1978, 2008. SUHAIMI N, RAMLI S, MALEK RA, AZIZ R, OTHMAN NZ, LENG OM, ESAWY M, GAMAL A, EL-ENSHASY H, Optimization of pectinase production by Aspergillus niger using Orange pectin based médium. J Chem Pharm Resea, v. 8, p. 259-268, 2016.

Como citar:

ROCHA, J. C.; ARAÚJO, J. S.; PAIVA, W. K. V.; FERREIRA, C. R. R.; SILVA, M. G. F.; SANTOS, E. S.; SOUSA JR, F. C.; "PECTINASE PRODUCTION BY ASPERGILLUS NIGER IOC 4003 BY SOLID-STATE FERMENTATION USING CAJA DEPULPING RESIDUE", p-2145-2148. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-PT.0568