Blucher Chemical Engineering Proceedings

Setembro 2018, vol.1, num.5 - XXII Congresso Brasileiro de Engenharia Química
Oral - Open Access Idioma principal | Segundo principal

ONLINE MONITORING OF Pichia pastoris METABOLISM THROUGH RESPIRATION

ONLINE MONITORING OF Pichia pastoris METABOLISM THROUGH RESPIRATION

PARIZOTTO, L; PESSOA Jr., A; TONSO, A

Oral:

The online respiration monitoring is a useful tool to have insights about the cell culture. Furthermore, it allows modifications during the process to improve yields. Cultures of Pichia pastoris modified to produce L-asparaginase, a chemotherapy enzyme, were evaluated by their oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER). The results indicated which process condition was beneficial to cell growth and that the production phase can be improved by increasing the inductor feed.

The online respiration monitoring is a useful tool to have insights about the cell culture. Furthermore, it allows modifications during the process to improve yields. Cultures of Pichia pastoris modified to produce L-asparaginase, a chemotherapy enzyme, were evaluated by their oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER). The results indicated which process condition was beneficial to cell growth and that the production phase can be improved by increasing the inductor feed.

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DOI: 10.5151/cobeq2018-CO.186

Referências bibliográficas
  • [1] Buba, Juliano Costa. 2018. “Produção de Protease Com Atividade Fibrinolítica Por Cultivo Submerso de Mucor
  • [2] Subtilissimus Em Biorreator.” Universidade de São Paulo. Available online:
  • [3] http://www.teses.usp.br/teses/disponiveis/3/3137/tde-28022018-133041/pt-br.php
  • [4] Effer, Brian, Sindy Cabarca, Guilherme Meira Lima, Ignácio Mogel, Adalberto Pessoa Jr., Jorge G. Farías, and
  • [5] Gisele Monteiro. 2017. “Recombinant Expression of L-Asparaginase from Dickeya Chrysanthemi in
  • [6] Yeast: Effect of Endogenous Signal Sequence and Histidine Tag in the Expression Protein.” Manuscript
  • [7] submitted for publication.
  • [8] Garcia-Ochoa, Felix, Emilio Gomez, Victoria E. Santos, and Jose C. Merchuk. 2010. “Oxygen Uptake Rate in
  • [9] Microbial Processes: An Overview.” Biochemical Engineering Journal 49 (3). Elsevier B.V.: 289–307.
  • [10] doi:10.1016/j.bej.2010.01.011.
  • [11] Invitrogen Corporation. 2002. “Pichia Fermentation Process Guidelines Overview,” 1–11.
  • [12] Schmidell, Willibaldo. 2001. “Agitação E Aeração Em Biorreatores.” In Biotecnologia Industrial: Volume 2 -
  • [13] Engenharia Bioquímica, 277–329. São Paulo: Edgard Blücher ltda.
Como citar:

PARIZOTTO, L; PESSOA Jr., A; TONSO, A; "ONLINE MONITORING OF Pichia pastoris METABOLISM THROUGH RESPIRATION", p-4815-4818. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-CO.186

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