Exposure Assessment of Dogs To Aflatoxins

Exposure Assessment of Dogs To Aflatoxins

Teixeira, Erika Mitsuo Kiyoko; Frehse, Michele; Freire, Roberta Lemos; Hirozawa, Melissa Tiemi; Bordini, Jaqueline Gozzi; Ono, Mario Augusto; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque

Abstract:

Aflatoxins are mycotoxins produced mainly by Aspergillus flavus, A.parasiticus and A.nomius which contaminate cereals and cereal products. Aflatoxins in feed are associated with toxic and carcinogenic effects in animals and can result in great economic losses. In dogs, aflatoxins can cause anorexia, depression, icterus, vomiting, diarrhea and sudden death. The aim of this study was to evaluate the exposure of dogs to aflatoxins through naturally contaminated feed. For this purpose occurrence of aflatoxins was in three feed types (Standard, Premium and Super Premium) intended for dogs (n=81), collected from the residence of the owners in Northern Paraná State, Brazil. Aflatoxins were analyzed by a reversed phase isocratic high performance liquid chromatographic system, using a C-18 Luna Phenomenex column and acetonitrile:water (25:75, v/v) as mobile phase. Aflatoxins were detected in 100 % dog feed samples. Aflatoxin levels (AFBAndlt;subAndgt;1Andlt;/subAndgt;+AFBAndlt;subAndgt;2Andlt;/subAndgt;+AFGAndlt;subAndgt;1Andlt;/subAndgt;+AFGAndlt;subAndgt;2Andlt;/subAndgt;) in Standard, Premium and Super Premium feed ranged from 0.16 to 5.39 µg/kg (mean 1.29 µg/kg), 0.21 to 6.16 µg/kg (mean 0.49 µg/kg) and 0.19 to 2.64 µg/kg (mean 0.53 µg/kg), respectively. The mean aflatoxin levels of Standard feed differed significantly from the Premium and Super Premium feed by the Kruskal-Wallis test (pAndamp;#706;0.05). In spite of the maximum allowed levels for aflatoxins have not been established for pet food, the maximum levels detected (6.16 µg/kg), were below the maximum tolerated limit (20 µg/kg) recommended by the Brazilian Association of Pet Products Industry (2013). The estimated mean daily intake of aflatoxins (0.013 µg/kg bw/day) for dogs was below the acceptable daily intake (20 µg/kg body weight/day) and the pet safe dietary level (6 µg/kg pet food), indicating that all the feed samples were considered safe for dogs concerning hepatotoxicity. Nevertheless a continuous monitoring of the feed producing chain is essential in order to minimize pet health hazards.

Aflatoxins are mycotoxins produced mainly by Aspergillus flavus, A.parasiticus and A.nomius which contaminate cereals and cereal products. Aflatoxins in feed are associated with toxic and carcinogenic effects in animals and can result in great economic losses. In dogs, aflatoxins can cause anorexia, depression, icterus, vomiting, diarrhea and sudden death. The aim of this study was to evaluate the exposure of dogs to aflatoxins through naturally contaminated feed. For this purpose occurrence of aflatoxins was in three feed types (Standard, Premium and Super Premium) intended for dogs (n=81), collected from the residence of the owners in Northern Paraná State, Brazil. Aflatoxins were analyzed by a reversed phase isocratic high performance liquid chromatographic system, using a C-18 Luna Phenomenex column and acetonitrile:water (25:75, v/v) as mobile phase. Aflatoxins were detected in 100 % dog feed samples. Aflatoxin levels (AFBAndlt;subAndgt;1Andlt;/subAndgt;+AFBAndlt;subAndgt;2Andlt;/subAndgt;+AFGAndlt;subAndgt;1Andlt;/subAndgt;+AFGAndlt;subAndgt;2Andlt;/subAndgt;) in Standard, Premium and Super Premium feed ranged from 0.16 to 5.39 µg/kg (mean 1.29 µg/kg), 0.21 to 6.16 µg/kg (mean 0.49 µg/kg) and 0.19 to 2.64 µg/kg (mean 0.53 µg/kg), respectively. The mean aflatoxin levels of Standard feed differed significantly from the Premium and Super Premium feed by the Kruskal-Wallis test (pAndamp;#706;0.05). In spite of the maximum allowed levels for aflatoxins have not been established for pet food, the maximum levels detected (6.16 µg/kg), were below the maximum tolerated limit (20 µg/kg) recommended by the Brazilian Association of Pet Products Industry (2013). The estimated mean daily intake of aflatoxins (0.013 µg/kg bw/day) for dogs was below the acceptable daily intake (20 µg/kg body weight/day) and the pet safe dietary level (6 µg/kg pet food), indicating that all the feed samples were considered safe for dogs concerning hepatotoxicity. Nevertheless a continuous monitoring of the feed producing chain is essential in order to minimize pet health hazards.

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DOI: 10.5151/foodsci-microal-047

Como citar:

Teixeira, Erika Mitsuo Kiyoko; Frehse, Michele; Freire, Roberta Lemos; Hirozawa, Melissa Tiemi; Bordini, Jaqueline Gozzi; Ono, Mario Augusto; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque; "Exposure Assessment of Dogs To Aflatoxins", p-473-474. In: Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene [=Blucher Food Science Proceedings, v.1, n.1]. São Paulo: Blucher, 2014.
ISSN 2359201X, DOI 10.5151/foodsci-microal-047