Blucher Chemical Engineering Proceedings

Setembro 2018, vol.1, num.5 - XXII Congresso Brasileiro de Engenharia Química
Oral - Open Access Idioma principal | Segundo principal

EFFECT OF METAL IONS ON CELLULASES ACTIVITIES OF BACILLUS SP SMIA-2 .

EFFECT OF METAL IONS ON CELLULASES ACTIVITIES OF BACILLUS SP SMIA-2 .

CRUZ, E; COSTA, E. A; MARTINS, M. L. L

Oral:

Many enzymes utilize divalent metal ions as co-factors for catalysis. In this work was studied the effect of metal ions on activity and stability of crude extracts containing cellulases, obtained from submerged cultures of Bacillus sp. SMIA-2 containing corn steep liquor, sugarcane bagasse and passion fruit rind flour. The activit of avicelase (avicelhydrolyzing enzymes) was stimulated by Co2+ and Ba+2 at 1mM concentrations, while the CMCase (carboxymethylcellulose-hydrolyzing enzymes) was stimulated mainly by Mn2+ , indicating that these ions had a functional role in the molecular structure of the cellulases of Bacillus sp SMIA-2.

Many enzymes utilize divalent metal ions as co-factors for catalysis. In this work was studied the effect of metal ions on activity and stability of crude extracts containing cellulases, obtained from submerged cultures of Bacillus sp. SMIA-2 containing corn steep liquor, sugarcane bagasse and passion fruit rind flour. The activit of avicelase (avicelhydrolyzing enzymes) was stimulated by Co2+ and Ba+2 at 1mM concentrations, while the CMCase (carboxymethylcellulose-hydrolyzing enzymes) was stimulated mainly by Mn2+ , indicating that these ions had a functional role in the molecular structure of the cellulases of Bacillus sp SMIA-2.

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DOI: 10.5151/cobeq2018-CO.117

Referências bibliográficas
  • [1] JUTURU V, WU JC, Microbial cellulases: Engineering, production and applications. Renew
  • [2] Sust Energ Rev 33, p. 188–203, 2014.
  • [3] KARMAKAR M, RAY RR, Current trends in research and application of microbial cellulases.
  • [4] Res J Microbiol v.6, p .41–53, 2011.
  • [5] KUHAD RC, GUPTA R, SINGH A, Microbial cellulases and their industrial
  • [6] applications.Enzym Res 2011.
  • [7] MILLER GL, Use of dinitrosalicylic acid reagent for determination of reducing sugars.Anal.
  • [8] Chem v.3, p. 426-428, 1959.
  • [9] NUNES AS, MARTINS, MLL, Isolation, properties and Kinetics of growth of a thermophilic
  • [10] Bacillus. Brasilian Journal of Microbiology, v.32,n.2, p. 271-275, 2001.
  • [11] OVIEDO C, RODRÍGUEZ J, The chelating agent under environmental scrutiny. Qum. Nova
  • [12] 26, p. 901–905, 2003.
  • [13] YANASE S, YAMADA R, KANEKO S, NODA H, HASUNUMA T, TANAKA T, Ethanol
  • [14] production from cellulosic materials using cellulase-expressing yeast. Biotechnol J, v.5, p. 449–
  • [15] 55, 2010.
Como citar:

CRUZ, E; COSTA, E. A; MARTINS, M. L. L; "EFFECT OF METAL IONS ON CELLULASES ACTIVITIES OF BACILLUS SP SMIA-2 .", p-4537-4540. In: . São Paulo: Blucher, 2018.
ISSN 23591757, DOI 10.5151/cobeq2018-CO.117

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