Setembro 2018 vol. 1 num. 5 - XXII Congresso Brasileiro de Engenharia Química

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CULTIVATION OF Bacillus thuringiensis var. israelensis H14 IN BIOREACTOR FOR BIOLOGICAL CONTROL OF Aedes aegypti LARVAE

MELO, L. F. A.; CABRAL, A. M.; MELO, A. C. A.; MELO-SANTOS, M. A. V.; FINKLER, L.; LUNA-FINKLER, C .L.;

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The aim of this study was the cultivation of Bacillus thuringiensisvar. israelensis (Bti) H14 for the biological control of Aedes aegypti. Bti cultureswere cultivated in a bioreactor, and the growth kinetics, toxin concentration andlarvicidal activity were evaluated. The results showed that the bacterialfermentates produced on average 0.62 g/L of toxin (LC50 2.25 mg/L and LC903.82 mg/L). Upon concentration of the biomass, the toxin yield was almostthree-fold higher (1.64 g/L), also resulting in an increase in the bioactivity(LC50 0.46 mg/L and LC90 0.84 mg/L). The fermentation conditions employedwere suitable for the cultivation of Bti, generating batches with highconcentration of cells, short time required for sporulation and adequateproduction of toxins with confirmed biolarvicidal activity.

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DOI: 10.5151/cobeq2018-PT.0577

Referências bibliográficas
  • [1] ARONSON AI, BECKMAN W, DUNN P, Bacillus thuringiensis and related insect pathogens. Microbiological Reviews, v. 50, n. 1, p. 1-24, 1986. BLÁZQUEZ A-B, SAIZ J-C, Neurological manifestations of Zika virus infection. World Journal of Virology, v. 5, n. 4, p. 135-143, 2016. DOI: 10.5501/wjv.v5.i4.135. BRADFORD MM, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, v. 72, n. 1-2, p. 248-254, 1976. DOI: 10.1016/0003-2697(76)90527-3. BRAGA IA, VALLE D, Aedes aegypti: histórico do controle no Brasil. Epidemiologia e Serviços de Saúde, v. 16, p. 113-118, 2007. DOI: 10.5123/S1679-49742007000200006. CAMPOS GS, BANDEIRA AC, SARDI SI, Zika virus outbreak, Bahia, Brazil. Emerging Infectious Diseases, v. 21, n. 10, p. 1885-1886, 2015. DOI: 10.3201/eid2110.150847. CHARLES J-F, Action de la δ endotoxine de Bacillus thuringiensis var. israelensis sur cultures de cellules de Aedes aegypti L. en microscopie eletronique. Annals of Microbiology, v. 134A, p. 365-381, 1983. CUNHA MP, LIMA JBP, BROGDON WG, MOYA GE, VALLE D, Monitoring of resistance to the pyrethroid cypermethrin in Brazilian Aedes aegypti (Diptera: Culicidae) populations collected between 2001 and 2003. Memórias do Instituto Oswaldo Cruz, v. 100, p. 441-444, 2005. DE BARJAC H, Une nouvelle variété de Bacillus thuringiensis très toxique pour les moustiques: B. thuringiensis var. israelensis sérotype H14. Comptes Rendus des Seances de L'Academie des Sciences, v. 286, p. 297-314, 1978. DE BARJAC H, LARGET-THIÉRY I, Characteristics of IPS-82 as standard for biological assay of Bacillus thuringiensis H-14 preparations. WHO Mimeograph Document, VBC/84.892, Geneva, Switzerland, 1984. ELSAYED EA, OTHMAN NZ, MALEK R, AWAD HM, WU K, AZIZ R, WADAAN MA, ENSHASY HAE, Bioprocess development for high cell mass and endospore production by Bacillus thuringiensis var israelensis in semi-industrial scale. Journal of Pure and Applied Microbiology, v. 8, n. 4, p. 2773-2783, 2014. LUNA CL, Avaliação de técnicas de separação fluido-sólido na produção de bioinseticidas a partir de Bacillus sphaericus e Bacillus thuringiensis var. israelensis. 2004. 202 f. Doctorate (Programa de Engenharia Química) - Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa de Engenharia (Coppe), Universidade Federal do Rio de Janeiro, Rio de Janeiro, 2004. MOUNSEF JR, SALAMEH D, AWAD MK, CHAMY L, BRANDAM C, LTEIF R, A simple method for the separation of Bacillus thuringiensis spores and crystals. Journal of Microbiological Methods, v. 107, p. 147-149, 2014. DOI: 10.1016/j.mimet.2014.10.003. NUNES ML, CARLINI CR, MARINOWIC D, KALIL NETO F, FIORI HH, SCOTTA MC, ZANELLA PLÁ, SODER RB, COSTA JCD, Microcephaly and Zika virus: a clinical and epidemiological analysis of the current outbreak in Brazil. Jornal de Pediatria, v. 92, n. 3, p. 230-240, 2016. DOI: 10.1016/j.jped.2016.02.009.
Como citar:

MELO, L. F. A.; CABRAL, A. M.; MELO, A. C. A.; MELO-SANTOS, M. A. V.; FINKLER, L.; LUNA-FINKLER, C .L.; "CULTIVATION OF Bacillus thuringiensis var. israelensis H14 IN BIOREACTOR FOR BIOLOGICAL CONTROL OF Aedes aegypti LARVAE", p. 2182-2185 . In: . São Paulo: Blucher, 2018.
ISSN 2359-1757, DOI 10.5151/cobeq2018-PT.0577

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