Contamination of Dog Feed by Fumonisins

Contamination of Dog Feed by Fumonisins

Teixeira, Erika Mitsuo Kiyoko; Frehse, Michele; Freire, Roberta Lemos; Hirozawa, Melissa Tiemi; Bordini, Jaqueline Gozzi; Ono, Mario Augusto; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque

Abstract:

Cereal grains used in feed production are often contaminated with toxic secondary metabolites produced by filamentous fungi (mycotoxins). Fumonisins are a group of mycotoxins produced mainly by Fusarium verticillioides and F. proliferatum. Although several analogues have been identified and characterized, fumonisin BAndlt;subAndgt;1Andlt;/subAndgt; (FBAndlt;subAndgt;1Andlt;/subAndgt;, FBAndlt;subAndgt;2Andlt;/subAndgt;, and FBAndlt;subAndgt;3Andlt;/subAndgt; occur at significant levels in naturally contaminated corn and corn-based products. Fumonisins in feed are associated with toxic effects such as interference with cell membrane metabolism, inhibition of sphingolipid metabolism and damage of several organs. The aim of this study was to evaluate the contamination of dog feed by fumonisins in Northern Paraná State, Brazil. Natural occurrence of fumonisins was evaluated in three feed types (Standard, Premium and Super Premium) intended for dogs (n=81), collected from the residence of the owners. Fumonisins were determined by a reverse-phase isocratic HPLC system, using methanol: 0.1 M NaHAndlt;subAndgt;2Andlt;/subAndgt;POAndlt;subAndgt;4Andlt;/subAndgt; (80:20, v/v) adjusted to pH 3.3 as mobile phase. FBAndlt;subAndgt;1Andlt;/subAndgt; and FBAndlt;subAndgt;2Andlt;/subAndgt; were detected in 72.8% and 51.9% of feed samples with levels ranging from 31.3 to 303.7 µg/kg and 36.1 to 972.2 µg/kg, respectively. Fumonisins (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) were detected in 77.6%, 72% and 42.9% of Standard, Premium and Super Premium feed, respectively. Most feed samples (66.7%) showed fumonisin (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) levels below 450 µg/kg. Mean FBs (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) levels were 272.43 µg/kg (Standard), 78.22 µg/kg (Premium), and 186.53 µg/kg (Super Premium). There was no significant difference in mean fumonisin levels among all the dog feed types by the Kruskal-Wallis test (pAndamp;#707;0.05). In spite of the maximum allowed levels for fumonisins (FBAndlt;subAndgt;1Andlt;/subAndgt; + FBAndlt;subAndgt;2Andlt;/subAndgt;) have not been established for pet food, the maximum levels detected (1014.72 µg/kg) were below the maximum tolerable limit (4000 µg/kg) recommended by the Brazilian Association of Pet Products Industry (2013). The estimated mean fumonisin daily intake (1.479 µg/kg bw/day) for dogs was below the acceptable daily intake (20 µg/kg body weight/day) and the pet safe dietary level (2000 µg/kg dog feed), indicating that all the feed samples were considered safe for dogs concerning fumonisins. Despite the low levels of contamination observed in this study, more studies are necessary to evaluate the chronic effects of fumonisins on dog health.

Cereal grains used in feed production are often contaminated with toxic secondary metabolites produced by filamentous fungi (mycotoxins). Fumonisins are a group of mycotoxins produced mainly by Fusarium verticillioides and F. proliferatum. Although several analogues have been identified and characterized, fumonisin BAndlt;subAndgt;1Andlt;/subAndgt; (FBAndlt;subAndgt;1Andlt;/subAndgt;, FBAndlt;subAndgt;2Andlt;/subAndgt;, and FBAndlt;subAndgt;3Andlt;/subAndgt; occur at significant levels in naturally contaminated corn and corn-based products. Fumonisins in feed are associated with toxic effects such as interference with cell membrane metabolism, inhibition of sphingolipid metabolism and damage of several organs. The aim of this study was to evaluate the contamination of dog feed by fumonisins in Northern Paraná State, Brazil. Natural occurrence of fumonisins was evaluated in three feed types (Standard, Premium and Super Premium) intended for dogs (n=81), collected from the residence of the owners. Fumonisins were determined by a reverse-phase isocratic HPLC system, using methanol: 0.1 M NaHAndlt;subAndgt;2Andlt;/subAndgt;POAndlt;subAndgt;4Andlt;/subAndgt; (80:20, v/v) adjusted to pH 3.3 as mobile phase. FBAndlt;subAndgt;1Andlt;/subAndgt; and FBAndlt;subAndgt;2Andlt;/subAndgt; were detected in 72.8% and 51.9% of feed samples with levels ranging from 31.3 to 303.7 µg/kg and 36.1 to 972.2 µg/kg, respectively. Fumonisins (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) were detected in 77.6%, 72% and 42.9% of Standard, Premium and Super Premium feed, respectively. Most feed samples (66.7%) showed fumonisin (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) levels below 450 µg/kg. Mean FBs (FBAndlt;subAndgt;1Andlt;/subAndgt;+FBAndlt;subAndgt;2Andlt;/subAndgt;) levels were 272.43 µg/kg (Standard), 78.22 µg/kg (Premium), and 186.53 µg/kg (Super Premium). There was no significant difference in mean fumonisin levels among all the dog feed types by the Kruskal-Wallis test (pAndamp;#707;0.05). In spite of the maximum allowed levels for fumonisins (FBAndlt;subAndgt;1Andlt;/subAndgt; + FBAndlt;subAndgt;2Andlt;/subAndgt;) have not been established for pet food, the maximum levels detected (1014.72 µg/kg) were below the maximum tolerable limit (4000 µg/kg) recommended by the Brazilian Association of Pet Products Industry (2013). The estimated mean fumonisin daily intake (1.479 µg/kg bw/day) for dogs was below the acceptable daily intake (20 µg/kg body weight/day) and the pet safe dietary level (2000 µg/kg dog feed), indicating that all the feed samples were considered safe for dogs concerning fumonisins. Despite the low levels of contamination observed in this study, more studies are necessary to evaluate the chronic effects of fumonisins on dog health.

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DOI: 10.5151/foodsci-microal-063

Como citar:

Teixeira, Erika Mitsuo Kiyoko; Frehse, Michele; Freire, Roberta Lemos; Hirozawa, Melissa Tiemi; Bordini, Jaqueline Gozzi; Ono, Mario Augusto; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque; "Contamination of Dog Feed by Fumonisins", p-499-500. In: Proceedings of the XII Latin American Congress on Food Microbiology and Hygiene [=Blucher Food Science Proceedings, v.1, n.1]. São Paulo: Blucher, 2014.
ISSN 2359201X, DOI 10.5151/foodsci-microal-063