fevereiro 2015 vol. 1 num. 2 - XX Congresso Brasileiro de Engenharia Química

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1 EXTRACTION OF AGGLUTININS FROM Arthrospira platensis BIOMASS IN THREE DIFFERENT BUFFERS

LIMA, K. L.; NASCIMENTO, C. O .; COSTA, R. M. P. B.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA;

Artigo:

Arthrospira platensis contains large amounts of proteins, vitamins, lipids and pigments with various biological functions, such as antitumor, antiviral, antimicrobial activities. The aim of this work was detected the presence of agglutinins in Arthrospira platensis biomass extracted in three different buffers. The harvested cells were concentrated by a centrifugation at 4,000 xg for 10 min, lyophilized and extracted under sonication in pure and in concentrations of 1:5 to 1:20 of 0.025M Tris-HCl-saline, pH 7.0; 0.02M saline-phosphate, pH 7.4; and 0.1M sodium acetate, pH, 5.5 buffers. For hemagglutination activity tests, in microtiter boards, extracts were added to rabbit erythrocytes. Positive results were obtained for hemagglutination activity only in saline-phosphate buffer (1:5, 1:10, and 1:20 dilutions) and sodium acetate buffer (1:5, and 1:10 dilutions) with titers of 2

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DOI: 10.5151/chemeng-cobeq2014-0592-24800-171812

Referências bibliográficas
  • [1] AMAO, Y. and NAKAMURA, N. 2006, Biohydrogen production with the light-harvesting function of grana Área temática: Processos Biotecnológicos 45 CASTENHOLZ, R.W. 200 Oxygenic photosynthetic bacteria. In: Boone D.R. and Castenholz R.W. (eds), Bergey’s Manual of Systematic Bacteriology (2nd ed.), Volume 1, Springer-Verlag, New York, pp. 473-600.Congress of Applied Algology, Czech Republic. CHU, C.Y.; HUANG, R.; LIN, L.P. Purification and characterization of a novel haemagglutinin from Chlorella pyrenoidosa.Journal of Industrial Microbiology and Biotechnology, v. 33, p. 967–973, 2006.
  • [2] CORREIA M.T.S., COELHO L.C.B.B. Purification of a glucose/mannose specific lectin, isoform 1, from seeds of Cratylia mollis mart (Camaratu Bean), Appl Biochem Biotech. 55 (1995) 261-273.
  • [3] DESIKACHARY, T.V. 1973, In: Carr, N.G. and Whitton, B.A. (eds.), Botanical Monographs, Blackwell Scientific. Publications: Oxford, London, Edinburgh, Melbourne, pp. 473–81 DINH, H. L.; HORI, K.; QUANG, N. H. Screening and preliminary characterization of hemagglutinins in Vietnamese marine algae. Journal Applied of Phycology, v. 21, p. 89–97, 2009.
  • [4] DOOLITTLE, W.F. 1982, In: Carr, N.G. and Whitton, B.A. (eds.), The Biology of Cyanobacteria, Botanical from Spirulina and colloidal platinum, Int. J. Hydro. Energy, 29, 39–42.
  • [5] FÁBREGAS, J.; LLOVO, J. Andamp; MUÑOZ, A. Hemagglutinins in red seaweeds. Botanica Marina, 28(12):517-520, 198
  • [6] HOICZYK, E. 2000, Gliding motility in cyanobacteria: observations and possible explanations, Arch. Microbiol., 174, 11–7.
  • [7] HORI, K.; MIYAZAWA, K.Andamp; Ito, K. Hemagglutinins in marine algae. Bulletin Japanese Society of Scientific Fisheries, 47(6):793-798, 1981.
  • [8] LIMA, H.C.; COSTA, F.H.F.; SAMPAIO, A.H.; NEVES, S.A.; BENEVIDES, N.M.B.; TEIXEIRA, D.I.A; ROGERS, D.J.; FREITAS, A.L.P. Induction and inhibition of human lymphocyte transformation by the lectin from the red marine algae Amansia multifida. Journal Applied of Phycology, v. 10, p. 153–162, 199
  • [9] LOSEVA, L.P. and Dardynskaya, I.V. 1993, Spirulina natural sorbent of radionucleides, 6th International Monographs, University of California Press: Berkeley and Los Angels, pp. 307–31.
  • [10] MUÑOZ, A.; LLOVO, J. Andamp; FABREGAS, J. Hemaglutininas de algas verdes. Acta Científica Compostelana, 22:873- 878, 1985.
  • [11] NEVES, S.A.; BARU, Y.M.D.; FREITAS, A.L.P.; BARREIRA, M.C.R. Neutrophil migration induced in vivo and vitro by marine algae lectins. Inflammation Research, v. 50, p. 486–490, 2001 Área temática: Processos Biotecnológicos 56 SAMPAIO, A.H; AINOUZ, I.L; FREITAS, A.L.P; BENEVIDES, N.M.B. Hemagutininas de Algas Marinhas. R. Bras. Fisiol. Veg., 5(2):171-177, 1993.
  • [12] SCHOSSER, U.G. Sammlung von Algenkulturen. Ber. Dtsch. Bot. Ges. v.95, p.181-276, 1982.
  • [13] TOMASELLI L, Maria Rosa P, Mario RT. On the cor-rect use of the Spirulina designation. Algol Stud 1996; 83:539-548.
  • [14] VAN TUERENHOUT, D. 2005, The Aztecs: New Perspectives, ABC-CLIO
Como citar:

LIMA, K. L.; NASCIMENTO, C. O .; COSTA, R. M. P. B.; PORTO, A. L. F.; BEZERRA, R. P.; MARQUES, D. A. VIANA; "1 EXTRACTION OF AGGLUTININS FROM Arthrospira platensis BIOMASS IN THREE DIFFERENT BUFFERS", p. 888-893 . In: Anais do XX Congresso Brasileiro de Engenharia Química - COBEQ 2014 [= Blucher Chemical Engineering Proceedings, v.1, n.2]. São Paulo: Blucher, 2015.
ISSN 2359-1757, DOI 10.5151/chemeng-cobeq2014-0592-24800-171812

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